Core--Recombinant viral gene transfer

核心--重组病毒基因转移

• 批准号: 6608687
• 负责人: COREY H MJAATVEDT
• 金额: $ 19.45万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6608687
• 关键词: biomedical facility; recombinant virus; transfection; transfection /expression vector

(Adapted from the Applicant's Abstract) The purpose of the core is to provide the resources and expertise for the efficient generation and delivery into embryos of recombinant expression viruses (adenovirus and retroviruses) containing genes of interest to the project investigators. (End of Abstract) This core provides the critical adeno-viral and retro-viral support for the projects in this proposal. Most of the projects propose to either use lineage tracing or expression of certain genes utilizing either adeno-viral or retro-viral transfection into mouse or chick embryo cultures. This core is central to the experiments outlined in the various projects. The principal investigator and co-investigators have experience in the adeno-viral and retro-viral techniques proposed. Their approach is straight-forward and appropriate. They will maintain cell lines necessary for these experiments and generate the high titer viral stocks necessary for the experiments. They propose to make the core laboratory available to the investigators for them to learn as well as provide this service. The plans to provide direct infection of whole embryos in culture (mouse and chicken) are reasonable, and the investigators have experience with such techniques. There are overall no glaring difficulties with the proposed approach. The types of experiments proposed are now rather routine and will be useful for the various experiments outlined in the individual projects. The ability to infect specific tissues in whole embryo cultures is rather innovative, although it has been used successfully by other investigators. Nevertheless, it is not a common skill and it is certainly a strength that this core will have that ability. The prinicipal investigator and co-investigators have good experience, as they have demonstrated, utilizing retro-viral and adno-viral techniques. They have done these sorts of experiments for other purposes successfully, and should have no difficulty in performing the tasks outlined. Dr. Kubalak has had experience with mouse embryo culture and this will certainly be an asset to the program project grant. The environment for the core is strong and they appear to already have all the necessary equipment and technology to provide the core services outlined. There are no problems with the environment. This is a very important core for this program project and appears to be designed appropriately without any major flaws. The core has many strengths and will be a critical one for most of the projects. The availability of Dr. Mikawa as a consultant adds furthur strength to the ability of this core to troubleshoot and be effective.

（改编自申请人摘要）核心的目的是提供 有效生成和交付的资源和专业知识， 重组表达病毒（腺病毒和逆转录病毒）的胚胎 包含项目研究人员感兴趣的基因。(End摘要） 该核心为免疫调节提供了关键的腺病毒和逆转录病毒支持。 项目在这个提案中。大多数项目建议使用沿袭 利用腺病毒或逆转录病毒追踪或表达某些基因 转染到小鼠或鸡胚胎培养物中。这个核心是 在不同的项目中进行实验。 主要研究者和合作研究者在腺病毒方面有经验， 和逆转录病毒技术的建议。他们的方法很直接 而且合适他们将维持这些实验所需的细胞系 并产生实验所需的高滴度病毒原液。他们 建议向研究者提供核心实验室，以便他们 学习并提供这项服务。提供直接感染的计划 在培养（小鼠和鸡）的整个胚胎是合理的， 调查人员有使用这种技术的经验。总的来说，没有 提出的方法存在明显的困难。 所提议的实验类型现在已相当常规，而且将是有用的 用于各个项目中概述的各种实验。的能力 感染整个胚胎培养物中的特定组织是相当创新的， 尽管它已被其他研究者成功地使用。然而，尽管如此， 这不是一个普通的技能，它肯定是一个力量，这个核心将 有这种能力。 主要研究者和共同研究者经验丰富，因为他们 已经证明，利用逆转录病毒和腺病毒技术。他们有 为了其他目的成功地做了这些实验，应该 在执行所述任务时没有困难。库巴拉克博士 小鼠胚胎培养的经验，这将肯定是一个资产， 计划项目补助金。 核心的环境是强大的，他们似乎已经拥有了所有的 提供所述核心服务所需的设备和技术。 环境没有问题。 这是一个非常重要的核心，为这个项目的项目，似乎是 设计得当，没有任何重大缺陷。核心有很多优点 对大多数项目来说都是至关重要的。的可用性博士。 作为顾问，三川进一步加强了这一核心的能力， 故障排除和有效。