"Core C" Recombinant Virus Core

“Core C”重组病毒核心

• 批准号: 10646256
• 负责人: ROLF F RENNE
• 金额: $ 13.26万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-09 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10646256
• 关键词: Acquired Immunodeficiency Syndrome; Archives; Bacteria; Biology; CRISPR interference; CRISPR/Cas technology; Cell Line; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; DNA Resequencing; Development; DsRed; Financial Support; Funding; Generations; Genes; Genetic Recombination; Genomics; Goals; Herpesviridae; Human Herpesvirus 4; Human Herpesvirus 8; Institution; Malignant Neoplasms; MicroRNAs; Mutagenesis; Mutagens; Mutation; Oncogenic; Phenotype; Protocols documentation; Publications; Recombinants; Research; Resource Sharing; Role; Services; Techniques; Training; Untranslated RNA; Viral; Viral Genes; Virus Diseases; cost efficient; design; experience; gammaherpesvirus; genome-wide; innovation; mutant; programs; recombinant virus; response

Summary The purpose of the Recombinant Virus Core C is to generate quality controlled recombinant herpesviruses for KSHV, EBV, and MHV68 (Projects 1, 2 and 3). The services of Core C will continue to aid the overall goal of the program project to comprehensively analyze the role of herpesvirus non-coding RNAs as well as studies on host cellular lncRNAs that are perturbed in response to viral infection. In addition to constructing recombinant viruses, Core C will perform genome-wide sequencing of viral mutants. Building upon years of experience in making a complete set of KSHV miRNA deletion mutants, our group has developed and implemented techniques for making bacmid-derived mutants in both KSHV and MHV68, and more recently also EBV. During the first funding period we designed, created and quality controlled more than 60 recombinant herpesviruses. Additional services and innovations are target enriched re-sequencing of latently infected producer cell lines for EBV and KSHV and applying innovative CRISPR technologies for mutagenesis.

概括 重组病毒核心 C 的目的是生成质量受控的重组疱疹病毒，用于 KSHV、EBV 和 MHV68（项目 1、2 和 3）。 Core C的服务将继续助力实现 全面分析疱疹病毒非编码RNA的作用以及研究的计划项目 宿主细胞 lncRNA 因病毒感染而受到干扰。除了构建重组 对于病毒，Core C 将对病毒突变体进行全基因组测序。凭借多年的经验 制作一套完整的KSHV miRNA缺失突变体，我们小组开发并实施了技术 用于在 KSHV 和 MHV68 以及最近的 EBV 中制备杆粒衍生突变体。第一次期间 资助期间，我们设计、创建并控制了 60 多种重组疱疹病毒。额外的 服务和创新的目标是对潜伏感染的 EBV 生产细胞系进行富集重测序， KSHV 并应用创新的 CRISPR 技术进行诱变。