"Core C" Recombinant Virus Core

摘要 重组病毒核心C的目的是产生质量可控的重组疱疹病毒用于 KSHV、EBV和MHV68(项目1、2和3)。核心C的服务将继续帮助实现以下总体目标 该项目旨在全面分析疱疹病毒非编码RNA的作用，并研究 宿主细胞对病毒感染的反应受到干扰的lncRNA。除了构建重组 病毒，核心C将对病毒突变进行全基因组测序。以多年的经验为基础 制作了一套完整的KSHV miRNA缺失突变体，我们团队开发并实施了技术 用于在KSHV和MHV68以及最近还在EBV中制造巴克米德衍生的突变体。在第一次 资助期内我们设计、创造并质量控制了60多种重组疱疹病毒。其他内容 服务和创新是针对EBV和潜伏感染的生产者细胞系的目标丰富的重新测序 KSHV和应用创新的CRISPR技术进行诱变。

Summary The purpose of the Recombinant Virus Core C is to generate quality controlled recombinant herpesviruses for KSHV, EBV, and MHV68 (Projects 1, 2 and 3). The services of Core C will continue to aid the overall goal of the program project to comprehensively analyze the role of herpesvirus non-coding RNAs as well as studies on host cellular lncRNAs that are perturbed in response to viral infection. In addition to constructing recombinant viruses, Core C will perform genome-wide sequencing of viral mutants. Building upon years of experience in making a complete set of KSHV miRNA deletion mutants, our group has developed and implemented techniques for making bacmid-derived mutants in both KSHV and MHV68, and more recently also EBV. During the first funding period we designed, created and quality controlled more than 60 recombinant herpesviruses. Additional services and innovations are target enriched re-sequencing of latently infected producer cell lines for EBV and KSHV and applying innovative CRISPR technologies for mutagenesis.