"Core C" Recombinant Virus Core

总结 重组病毒核心C的目的是产生质量受控的重组疱疹病毒， KSHV、EBV和MHV 68（项目1、2和3）。核心C的服务将继续帮助实现 综合分析疱疹病毒非编码RNA的作用以及研究 宿主细胞lncRNA响应于病毒感染而受到干扰。除了构建重组 核心C将对病毒突变体进行全基因组测序。凭借多年的经验， 为了制造一套完整的KSHV miRNA缺失突变体，我们的团队开发并实施了技术， 用于在KSHV和MHV 68以及最近的EBV中制备杆粒衍生的突变体。在第一 在资助期内，我们设计、创造和质量控制了60多种重组疱疹病毒。额外 服务和创新是对潜伏感染的EBV生产细胞系进行靶向富集重测序， KSHV和应用创新的CRISPR技术进行诱变。

Summary The purpose of the Recombinant Virus Core C is to generate quality controlled recombinant herpesviruses for KSHV, EBV, and MHV68 (Projects 1, 2 and 3). The services of Core C will continue to aid the overall goal of the program project to comprehensively analyze the role of herpesvirus non-coding RNAs as well as studies on host cellular lncRNAs that are perturbed in response to viral infection. In addition to constructing recombinant viruses, Core C will perform genome-wide sequencing of viral mutants. Building upon years of experience in making a complete set of KSHV miRNA deletion mutants, our group has developed and implemented techniques for making bacmid-derived mutants in both KSHV and MHV68, and more recently also EBV. During the first funding period we designed, created and quality controlled more than 60 recombinant herpesviruses. Additional services and innovations are target enriched re-sequencing of latently infected producer cell lines for EBV and KSHV and applying innovative CRISPR technologies for mutagenesis.