CORE--NUCLEIC ACID FACILITY

核心--核酸设施

• 批准号: 6573826
• 负责人: PONZY LU
• 金额: $ 22.86万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6573826
• 关键词: DNA; RNA; biomedical facility; biomedical resource; neoplasm /cancer genetics; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides

The Nucleic Acid Facility has been continuously approved and funded by the NCI since 1984. The availability of both routine oligonucleotide synthesis and the ability to consult on and perform innovative synthesis projects makes this core highly valuable to Cancer Center members. Ponzy Lu, PhD, Professor of Chemistry, has dir4ected this Shared Resource since its inception. Xiaolin Zhang, PhD, has served as Technical Director since 1994. At the time of the last competitive renewal, the Facility received "excellent to outstanding" merit. The Nucleic Acid Facility has remained at the forefront of synthetic technology through leadership and scientific expertise of Drs. Lu and Zhang. The Directors assist researchers by exploring novel avenues of oligonucleotide modification and nucleic acid derivatives for basic science as well as translational research applications. This contribution is best exemplified in the Nucleic Acid Facility's inclusion as a core resource in a recently-funded NCI Program Project Grant to explore the effects of antisense oligonucleotides (Gewirtz, PI), and the use of its scientific and technical expertise to supply fluorinated gancyclovir for use as a PET probe in order to track in situ gene expression by gene therapy vectors (Eck and Alavi) Total facility usage has increased 152% during the current project period. Usage by Cancer Center members has increased 296%. The percentage of usage by Cancer Center members has also increased, from 47% in 1993 to 75% in 1998, and the percentage of usage by Cancer Center members with peer-reviewed funding has increased, from 33% in 1993 to 74% in 1998.

自1984年以来，核酸库一直得到NCI的批准和资助。常规寡核苷酸合成的可用性以及咨询和执行创新合成项目的能力使这一核心对癌症中心成员具有很高的价值。化学教授庞兹·卢博士自这个共享资源建立以来一直在指导它。张晓林博士自1994年起担任技术总监。在上一次竞争性续签时，该机制获得了“优秀到杰出”的成绩。由于卢博士和张博士的领导和科学专长，核酸设备一直处于合成技术的前沿。主任们通过探索用于基础科学和翻译研究应用的寡核苷酸修饰和核酸衍生物的新途径来协助研究人员。这一贡献的最好例证是，核酸设施作为核心资源被纳入最近资助的NCI计划项目赠款，以探索反义寡核苷酸(Gewirtz，PI)的影响，并利用其科学和技术专长提供氟化更昔洛韦作为PET探针，以便通过基因治疗载体(Eck和Alavi)跟踪原位基因表达。在本项目期间，总设施使用量增加了152%。癌症中心成员的使用量增加了296%。癌症中心成员的使用百分比也有所增加，从1993年的47%增加到1998年的75%，癌症中心成员使用同行审查资金的百分比也从1993年的33%增加到1998年的74%。