CORE--MOLECULAR GENETICS

核心--分子遗传学

• 批准号: 6565010
• 负责人: MARK LEPPERT
• 金额: $ 23.8万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6565010
• 关键词: biomedical facility; familial hypertension; genetic markers; molecular genetics; single nucleotide polymorphism

DESCRIPTION: (Adapted from the applicant's abstract) Genotypes for linkage and association were carried out in support of investigators in the current SCOR. A total of 41 separate marker systems from 21 genes were run. In all, 6,647 genotypes were obtained for this study. As new samples were arriving at the Core throughout the course of the grant, not all of the samples have been typed for all marker systems, however, an effort was made to complete typing on the new samples for key marker systems that yielded promising results in the original analysis. The Molecular Genetics core currently is completing the genotyping on the last group of a total of 214 sibling pair samples and for association studies and an additional 120 singleton samples for a total of 434 DNA samples. Linkage markers close to candidate genes as well as single nucleotide polymorphism (SNPs) within the candidate genes were run. Linkage markers were carried out using a radioactive/acrylamide detection system. SNPs were detected with either mass spectrometry, the allelic discrimination system of the Perkin-Elmer ABI Prism 7700, or restriction enzyme digestion followed by resolution on agarose gels.

产品说明：（改编自申请人的摘要）为支持当前SCOR中的研究者，进行了连锁和关联的基因型分析。 运行了来自21个基因的总共41个单独的标记系统。 在这项研究中，总共获得了6，647个基因型。 在整个赠款过程中，由于新样品到达核心，并非所有样品都对所有标记系统进行了分型，然而，我们努力完成了对新样品的分型，以获得在原始分析中产生有希望结果的关键标记系统。 分子遗传学核心目前正在完成最后一组共214个同胞对样本的基因分型，并进行关联研究，以及另外120个单细胞样本，共434个DNA样本。 运行接近候选基因的连锁标记以及候选基因内的单核苷酸多态性（SNP）。 使用放射性/丙烯酰胺检测系统进行连锁标记。 SNPs的检测与质谱法，等位基因识别系统的Perkin-Elmer ABI棱镜7700，或限制性内切酶消化，然后在琼脂糖凝胶上的分辨率。