CORE--MOLECULAR GENETICS

核心--分子遗传学

• 批准号: 7010662
• 负责人: MARK LEPPERT
• 金额: $ 18.21万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7010662
• 关键词: biomedical facility; familial hypertension; genetic markers; molecular genetics

DESCRIPTION: (Adapted from the applicant's abstract) Genotypes for linkage and association were carried out in support of investigators in the current SCOR. A total of 41 separate marker systems from 21 genes were run. In all, 6,647 genotypes were obtained for this study. As new samples were arriving at the Core throughout the course of the grant, not all of the samples have been typed for all marker systems, however, an effort was made to complete typing on the new samples for key marker systems that yielded promising results in the original analysis. The Molecular Genetics core currently is completing the genotyping on the last group of a total of 214 sibling pair samples and for association studies and an additional 120 singleton samples for a total of 434 DNA samples. Linkage markers close to candidate genes as well as single nucleotide polymorphism (SNPs) within the candidate genes were run. Linkage markers were carried out using a radioactive/acrylamide detection system. SNPs were detected with either mass spectrometry, the allelic discrimination system of the Perkin-Elmer ABI Prism 7700, or restriction enzyme digestion followed by resolution on agarose gels.

描述：（改编自申请人的摘要）为了支持当前SCOR的研究人员，进行了连锁和关联的基因型研究。共运行了来自21个基因的41个独立的标记系统。本研究共获得6647个基因型。随着新样品在整个资助过程中到达核心，并不是所有的样品都已经为所有的标记系统进行了打字，然而，我们努力完成对关键标记系统的新样品的打字，这些新样品在原始分析中产生了有希望的结果。分子遗传学核心目前正在完成对214个兄弟姐妹样本中的最后一组样本的基因分型和关联研究，以及另外120个单例样本，总共434个DNA样本。对候选基因附近的连锁标记和候选基因内的单核苷酸多态性进行分析。采用放射性/丙烯酰胺检测系统进行连锁标记。采用质谱法、Perkin-Elmer ABI Prism 7700等位基因识别系统或限制性内切酶切后琼脂糖凝胶分离检测snp。