Application of novel cellular target engagement technologies to interrogate the IL23R-JAK/TyK2-STAT signalling pathway

应用新型细胞靶点接合技术来探究 IL23R-JAK/TyK2-STAT 信号通路

• 批准号: 2106690
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2106690
• 关键词: Application; novel; cellular; target; engagement

Many biological processes are predicated on the binding of endogenous ligands and other protein-protein interactions that form part of a signaling cascade. Understanding the core components and interactions of these signaling cascades is still elusive for a number of cellular mechanisms with limited tools available to aid deconvolution. This study seeks to utilise the NanoBRET Tracer and protein-protein interaction (PPI) technology to target and better understand elements of a known signaling pathway (IL23R/JAK family/STAT) in a cellular context.

许多生物过程都是基于内源性配体的结合和其他蛋白质-蛋白质相互作用，形成信号级联的一部分。理解这些信号级联的核心成分和相互作用对于许多细胞机制来说仍然是难以捉摸的，可用的工具有限，可以帮助反卷积。本研究旨在利用NanoBRET示踪剂和蛋白-蛋白相互作用（PPI）技术来靶向和更好地理解细胞背景下已知信号通路（IL23R/JAK家族/STAT）的元素。