Mobility, microbial transformation & toxicity of NAPL

该项目的总体目的是评估在地下土壤和沉积物系统中溢出或处置的“煤焦油”和“Transformer油”中所含多环芳烃和多氯联苯的环境流动性、生物可处理性和表观遗传毒性。本研究将探讨：（1）混合非水相液体（NAPL）组分的吸附、封存和解吸过程，作为控制其环境迁移性的因素，与土壤和沉积物有机质的化学结构和性质有关;（二）土著微生物聚生体的生态学和生理学与污染物对微生物转化的亲和力之间的关系，作为控制其生物可处理性;以及，（iii）这些过程对表观遗传毒性的影响，作为衡量生物处理前后天然多孔介质中NAPL污染物的混合有机溢出物的毒性效应的措施。所获得的数据将解决基于风险的终点作为绝对清除目标的替代方案，用于修复受污染的土壤和沉积物的有效性。我们建议评估的吸附/解吸行为的NAPL相关的多环芳烃和多氯联苯的一系列表征土壤具有不同的成岩历史，因此化学和结构不同的天然有机质（NOM）组成。多环芳烃和多氯联苯的NAPL阶段，这些土壤和随后的封存和解吸的吸附将进行研究，以模拟他们的NAPL阶段，这些土壤和他们随后的封存和解吸将进行研究，以刺激他们的命运在“现实世界”NAPL污染的溢出和处置场地。多环芳烃和多氯联苯的微生物降解，无论是在NAPL和在土壤和沉积物固相污染的土著微生物财团的这些生理结构的可用性。混合有机溢出物的表观遗传毒性将通过连接细胞间通信的间隙来测量，作为评估受螯合和微生物转化过程影响的总体毒性降低的标准。

The overall purpose of this project is to assess the environmental mobility, biotreatability, and epigenetic toxicity of PAH's and PCB's contained in "coal tars" and "transformer oils" spilled or disposed of in subsurface soil and sediment systems. Th proposed research will examine: (i) sorption, sequestration, and desorption processed of mixed non-aqueous phase liquid (NAPL) components as factors controlling their environmental mobility in relation to the chemical structure and character of soil and sediment organic matter; (ii) relationships between the ecology and physiology of indigenous microbial consortia and the affinity of contaminants to microbial transformation as factors controlling their biotreatability; and, (iii) the impact of these processes on epigenetic toxicity as a measure of the toxic effects of mixed organic spills of NAPL contaminants in natural porous media prior to and after biotreatment. The data obtained will address the efficacy of risk-based endpoints as alternatives to absolute cleanup goals for the remediation of contaminated soils and sediments. We propose to evaluate the sorption/desorption behavior of NAPL-associated PAHs and PCBs for a range of well- characterized soils have different diagenetic histories and thus chemically and structurally different natural organic matter (NOM) compositions. The sorption of PAHs and PCBs from NAPL phase to these spoils and their subsequent sequestration and desorption will be studied to simulate their NAPL phase to these soils and their subsequent sequestration and desorption will be studied to stimulate their fate in "real-world" NAPL contaminated spill and disposal sites. The availability of PAHs and PCBs to microbial degradation both in the NAPL and in the soil and sediment solid phases contaminated by these physiological structures of indigenous microbial consortia. The epigenetic toxicity of mixed organic spills will be measured by a gap of junctional intercellular communication as a criteria for assessment of the reduction in overall toxicity effected by sequestration and microbial transformation processes.