Mobility, microbial transformation & toxicity of NAPL

该项目的总体目的是评估地下土壤和沉积物系统中泄漏或处置的“煤焦油”和“变压器油”中含有的多环芳烃和多氯联苯的环境流动性、生物可处理性和表观遗传毒性。这项拟议的研究将审查：(I)作为控制其环境流动性的因素处理的混合非水相液(NAPL)组分的吸附、隔离和解吸与土壤和沉积物有机质的化学结构和特征有关；(Ii)作为控制其可生物处理性的因素的本地微生物协会的生态和生理以及污染物对微生物转化的亲和力之间的关系；以及(Iii)作为生物处理前后天然多孔介质中NAPL污染物混合有机溢出的毒性影响的衡量标准，这些过程对表观遗传毒性的影响。所获得的数据将讨论以风险为基础的终点作为绝对清理目标的替代方案在修复受污染土壤和沉积物方面的效力。我们建议评估与NAPL相关的多环芳烃和多氯联苯的吸附/解吸行为，这些土壤具有不同的成岩历史，因此具有不同的化学和结构的天然有机物(NOM)组成。将研究多环芳烃和多氯联苯从NAPL相到这些废物的吸附以及随后的封存和解吸，以模拟它们在这些土壤中的NAPL相，并将研究它们随后的封存和解吸，以模拟它们在真实世界中的NAPL污染泄漏和处置场所的命运。多环芳烃和多氯联苯在NAPL和土壤中的微生物降解有效性以及被土著微生物群落的这些生理结构污染的沉积物固相中的微生物降解性。混合有机泄漏的表观遗传毒性将通过连接细胞间通讯的差距来衡量，作为评估隔离和微生物转化过程所影响的总体毒性降低的标准。

The overall purpose of this project is to assess the environmental mobility, biotreatability, and epigenetic toxicity of PAH's and PCB's contained in "coal tars" and "transformer oils" spilled or disposed of in subsurface soil and sediment systems. Th proposed research will examine: (i) sorption, sequestration, and desorption processed of mixed non-aqueous phase liquid (NAPL) components as factors controlling their environmental mobility in relation to the chemical structure and character of soil and sediment organic matter; (ii) relationships between the ecology and physiology of indigenous microbial consortia and the affinity of contaminants to microbial transformation as factors controlling their biotreatability; and, (iii) the impact of these processes on epigenetic toxicity as a measure of the toxic effects of mixed organic spills of NAPL contaminants in natural porous media prior to and after biotreatment. The data obtained will address the efficacy of risk-based endpoints as alternatives to absolute cleanup goals for the remediation of contaminated soils and sediments. We propose to evaluate the sorption/desorption behavior of NAPL-associated PAHs and PCBs for a range of well- characterized soils have different diagenetic histories and thus chemically and structurally different natural organic matter (NOM) compositions. The sorption of PAHs and PCBs from NAPL phase to these spoils and their subsequent sequestration and desorption will be studied to simulate their NAPL phase to these soils and their subsequent sequestration and desorption will be studied to stimulate their fate in "real-world" NAPL contaminated spill and disposal sites. The availability of PAHs and PCBs to microbial degradation both in the NAPL and in the soil and sediment solid phases contaminated by these physiological structures of indigenous microbial consortia. The epigenetic toxicity of mixed organic spills will be measured by a gap of junctional intercellular communication as a criteria for assessment of the reduction in overall toxicity effected by sequestration and microbial transformation processes.