Mobility, microbial transformation & toxicity of NAPL

该项目的总体目的是评估泄漏或处置于地下土壤和沉积物系统中的“煤焦油”和“变压器油”中所含多环芳烃和多氯联苯的环境迁移性、生物可处理性和表观遗传毒性。拟议的研究将研究：（i）混合非水相液体（NAPL）组分的吸附、封存和解吸过程作为控制其与土壤和沉积物有机质的化学结构和特征相关的环境迁移性的因素； (ii) 本土微生物群落的生态学和生理学与污染物与微生物转化的亲和力之间的关系作为控制其生物处理能力的因素； (iii) 这些过程对表观遗传毒性的影响，作为生物处理前后 NAPL 污染物混合有机溢出物在天然多孔介质中的毒性影响的衡量标准。获得的数据将解决基于风险的终点的有效性，作为修复受污染土壤和沉积物的绝对清理目标的替代方案。我们建议评估一系列具有不同成岩历史、因此化学和结构上不同的天然有机质 (NOM) 组成的明确土壤中 NAPL 相关 PAH 和 PCB 的吸附/解吸行为。将研究 NAPL 相中 PAH 和 PCB 对这些废弃物的吸附及其随后的封存和解吸，以模拟它们在这些土壤中的 NAPL 相，并将研究它们随后的封存和解吸，以模拟它们在“真实世界”NAPL 污染泄漏和处置场中的命运。 NAPL 以及被本土微生物群落的这些生理结构污染的土壤和沉积物固相中 PAH 和 PCB 对微生物降解的有效性。混合有机泄漏物的表观遗传毒性将通过细胞间连接通讯的间隙来测量，作为评估封存和微生物转化过程影响的总体毒性降低的标准。

The overall purpose of this project is to assess the environmental mobility, biotreatability, and epigenetic toxicity of PAH's and PCB's contained in "coal tars" and "transformer oils" spilled or disposed of in subsurface soil and sediment systems. Th proposed research will examine: (i) sorption, sequestration, and desorption processed of mixed non-aqueous phase liquid (NAPL) components as factors controlling their environmental mobility in relation to the chemical structure and character of soil and sediment organic matter; (ii) relationships between the ecology and physiology of indigenous microbial consortia and the affinity of contaminants to microbial transformation as factors controlling their biotreatability; and, (iii) the impact of these processes on epigenetic toxicity as a measure of the toxic effects of mixed organic spills of NAPL contaminants in natural porous media prior to and after biotreatment. The data obtained will address the efficacy of risk-based endpoints as alternatives to absolute cleanup goals for the remediation of contaminated soils and sediments. We propose to evaluate the sorption/desorption behavior of NAPL-associated PAHs and PCBs for a range of well- characterized soils have different diagenetic histories and thus chemically and structurally different natural organic matter (NOM) compositions. The sorption of PAHs and PCBs from NAPL phase to these spoils and their subsequent sequestration and desorption will be studied to simulate their NAPL phase to these soils and their subsequent sequestration and desorption will be studied to stimulate their fate in "real-world" NAPL contaminated spill and disposal sites. The availability of PAHs and PCBs to microbial degradation both in the NAPL and in the soil and sediment solid phases contaminated by these physiological structures of indigenous microbial consortia. The epigenetic toxicity of mixed organic spills will be measured by a gap of junctional intercellular communication as a criteria for assessment of the reduction in overall toxicity effected by sequestration and microbial transformation processes.