Core--Digital imaging

体内和细胞成像共享资源包括有价值的现代工具，这些工具将作为常规服务提供给基因治疗中心成员。服务包括用多色荧光显微镜对活体和固定样本进行三维细胞和组织数字成像，以及体细胞显微注射。基于高分辨率成像技术，包括DeltaVision去卷积显微镜和细胞和组织的体积三维渲染。这些服务对于这个新的基因治疗中心的个别实验室项目和方案内研究项目至关重要，并将利用我们以前在这些方法方面的经验。在这项服务中，感染病毒的动物组织用立体组织成像技术进行免疫荧光染色。这项服务将能够对潜在宿主对这些组织中病毒的免疫反应进行成像分析，以帮助评估安全性。被病毒感染的培养细胞可以成像为经历变化的活细胞或免疫荧光试剂染色的固定样本，用于三维结构和功能分析。基因治疗中心的几名研究人员已经利用了在感染病毒载体后对活细胞和固定细胞进行成像的设施。由于我们中心的研究目标和努力涉及用于基因治疗的分子和细胞功能以及基因和病毒在细胞内的定位及其安全性，该资源承诺为这些基于细胞、组织和动物的研究提供具有成本效益的支持。资源人员将与项目调查人员交互，以访问加州大学圣迭戈分校提供的组织学、显微镜和图像分析资源。通过研究动物组织和细胞效应，这一资源将有助于了解病毒的生物学活性和动物组织中潜在的安全特征。通过使用这种资源来成像病毒颗粒和蛋白质在细胞和动物模型中的进入和命运，关于这些病毒的作用机制的详细信息将是可能的。

The In Vivo and Cellular Imaging Shared Resource comprises valuable and modern tools which are to be made available as routine services to Gene Therapy Center Members. Services include 3-dimensional cell and tissue digital imaging with multi-color fluorescence microscopy for both living and fixed samples and somatic cell microinjection. Based upon high resolution imaging techniques, including a DeltaVision deconvolution microscope and volumized 3-dimensional renderings of cells and tissues. These services have been crucial for individual lab projects and intra-programmatic research projects in this new Gen Therapy Center and will take advantage of our previous experience with these approaches. In this service, animal tissues infected with viral dimensional tissue imaging with immunofluorescence staining. The service will be able to provide imaging analysis of the potential host's immunological response to the virus in these tissues to help evaluate safety. Cells in culture infected with viral agents can be imaged as live cells undergoing changes or as fixed samples stained with immunofluorescent reagents for 3-dimensional structural and functional analysis. Several investigators in the Gene Therapy Center have already made use of the facility in which living and fixed cells have been imaged following infection with virus vectors. As research goals and efforts within our Center address the molecular and cellular functions and intracellular localization of genes and viruses to be used in gene therapy and their safety, this resource promises to provide cost-effective support for these cellular, tissue and animal-based studies. Resource personnel will interface with program investigators to enable access to the histology microscopy and image analysis resources available at UCSD. By examining both animal tissues and cellular effects, this resource will facilitate an understanding of both the biological activities of the viruses and potential safety features in animal tissues. By use of this resource to image the entry and fate of viral particles and proteins in cells and animal models, detailed information regarding the mechanism of action of these viruses will be possible.