Digital Imaging Core

数字成像核心(核心A)包括有价值的现代工具，这些工具将作为 为项目参与者提供常规服务。核心A的主要目标是提供统一的服务来支持计划 研究人员验证被转导的心脏细胞和组织。验证包括评估基因水平 表达，细胞间表达的可变性，以及对转导细胞的结构或功能的影响 基因表达的改变。服务包括具有多颜色的三维细胞和组织数字成像 活的和固定的样品的荧光显微镜。自上次审查以来，核心A已演变为包括 获得适合活细胞成像的多光子和多光谱共聚焦显微镜，以及活体内 用于活体动物成像的共焦系统。该计划的许多组成部分都涉及到对基因表达的分析 细胞和组织，因此这一核心将被广泛利用。基于高分辨率成像技术， 包括DeltaVision去卷积显微镜系统和Unix工作站，这些服务包括 定量图像分析、延时显微镜和细胞和组织的体积三维渲染。 通过与圣地亚哥的VisLab的互动，Core A的图像分析得到了极大的增强 超级计算机中心，使用NPACI可扩展可视化工具。这些工具允许实时 探索3-D中3~4个细胞成分的特殊位置这些服务对于 这一新计划中的个人实验室项目和项目内研究项目。在这项服务中，动物 转基因表达后的组织可以在几个水平上进行分析，包括大体病理和3- 免疫荧光染色的立体组织成像。该服务将能够提供成像 分析目标组织的各个方面，包括转基因特定细胞类型的鉴定 表达、组织炎症反应、由此产生的组织组织以及细胞基因的任何变化 表情或肌节的结构组织。在培养中被转导的细胞可以被成像为显示 时间关系或作为固定样本，用免疫荧光试剂染色，用于随后的3- 空间结构分析。随着我们计划中的研究目标和努力解决分子和 内源性和转导基因的细胞功能，这一资源承诺提供具有成本效益的支持 用于这些基于细胞、组织和动物的研究。

The Digital Imaging Core (Core A) comprises valuable and modem tools, which are to be made available as routine services to program participants. A major goal of Core A is to provide uniform services to enable Program investigators to validate transduced cardiac cells and tissues. Validation includes assessing level of gene expression, cell-to-cell variability in expression, and structural or functional consequences to transduced cells of the altered gene expression. Services include 3-dimensional cell and tissue digital imaging with multi-color fluorescence microscopy for both living and fixed samples. Since the last review, Core A has evolved to include access to a multi-photon and a multi-spectral confocal microscope fitted for live-cell imaging, and an intra-vital confocal system for live animal imaging. Many components of this Program involve analysis of gene expression in cells and tissues, and as such this Core will be widely utilized. Based upon high resolution imaging techniques, including a DeltaVision deconvolution microscope system and UNIX workstations, these services include quantitative image analysis, time-lapse microscopy and volume 3-dimensional renderings of cells and tissues. Through Core A image analysis is greatly enhanced through interactions with the VisLab at the San Diego Supercomputer Center, with use of NPACI Scalable Visualization Tools. These tools allow for real-time exploration of the special locations of 3 or 4 cellular components in 3-D. These services have been crucial for individual lab projects and intra-programmatic research projects in this new program. In this service, animal tissues following transgene expression can be analyzed at several levels, including gross pathology and 3- dimensional tissue imaging with immunofluorescence staining. The service will be able to provide imaging analysis of various aspects of the targeted tissues, including specific cell type identification of transgene expression, tissue inflammatory responses, resultant tissue organization, and any changes in cellular gene expression or sarcomeric structural organization. Cells transduced in culture can be imaged as live cells revealing temporal relationships or as fixed samples, stained with immunofluorescent reagents for subsequent 3- dimensional structural analysis. As research goals and efforts within our Program address the molecular and cellular functions of endogenous and transduced genes, this resource promises to provide cost-effective support for these cellular, tissue and animal-based studies.