Cell Imaging, Analysis and Microinjection

细胞成像，分析和显微注射核心（CIAMC）包括有价值的现代工具， 可作为计划项目成员的常规服务。所提供的服务对以下方面至关重要： 个别实验室项目和方案内研究。CIAMC成立于2000年， 以满足这一领域日益增长的调查需求。CIAMC已经发展到现在， Moores UCSD Digital Imaging Shared Resource（DISR）中的显微镜资源。我们 在此申请中，我建议资助一名兼职研究助理，为PPG提供专门的支持 成员1）提供标准化的培训，在使用这些三维荧光显微镜和 体细胞显微注射系统，2）在该PPG内训练个体以充分利用 增强的科学可视化软件，在我们的小组，和3）提供动手帮助的使用 这些显微镜。我们的设施的一个独特之处是与VisLab的计算机科学家合作 圣地亚哥超级计算机中心（SDSC）他们创造了增强3D的新工具 细胞的可视化，基于网络的数据处理，并利用了几乎无限的 SDSC的计算能力，以便于存储和分析非常大的数据集 (http：//vis.sdsc.edu/research/cancer.html）。随着频率的增加，目前的PPG研究人员正在进行 使用这些增强功能，拟议的CIAMC将促进PPG成员的能力， 及时使用这些服务。

The Cell Imaging, Analysis, & Microinjection Core (CIAMC) comprises valuable, modern tools that are made available as routine services to Program Project members. The services provided have been crucial for individual laboratory projects, and intra-programmatic research. The CIAMC was established in the original application to meet the growing needs of the investigators in this area. The CIAMC has evolved to now take advantage of microscopy resources in the Moores UCSD Digital Imaging Shared Resource (DISR). We propose in this application to fund a part-time research assistant to provide dedicated support to PPG members by 1) providing standardized training in the use of these 3-D fluorescence.microscopes and the somatic cell microinjection system, 2) training individuals within this PPG to take full advantage of the enhanced scientific visualization software available in our group, and 3) providing hands-on help with the use of these microscopes. A unique feature of our facility is its partnership with computer scientists at the VisLab in the San Diego Supercomputer Center (SDSC). They have created new tools for enhanced 3-D visualization of cells, web-based data processing, and have made use of the virtually unlimited computational power of the SDSC to facilitate storage and analysis of very large data sets (http://vis.sdsc.edu/research/cancer.html). With increasing frequency, current PPG investigators are making use of these enhanced features, and the proposed CIAMC will facilitate the ability of PPG members to access these services in a timely manner.