CORE--DIGITAL IMAGING AND CELL MICROINJECTION

数字成像和细胞显微注射(DICM)共享资源包括有价值的现代工具，这些工具作为常规服务提供给癌症中心成员。服务包括高分辨率数字去卷积显微镜、数字表观荧光和明场显微镜、定量图像分析、细胞显微注射和随后的生物分析、照相制作和培训以及咨询。这些服务对个别实验室项目、方案内研究和方案间研究至关重要。随着我们中心内针对我们的分子和细胞功能以及与癌细胞发生或生长调节有关的基因的细胞内定位的研究目标和努力越来越频繁地出现，该中心已采取措施为这些基于细胞和组织的研究提供经济有效的支持。这一演变源于这样一个事实，即研究人员，如我们癌症生物学和临床调查中的那些人。项目，一直在寻找可能对此负责的候选基因。这强化了这样一个事实，即解决这些基因功能的非常重要的任务往往是速率限制，这是上述项目以及我们中心的其他项目的一个基本特征。由于候选基因功能的线索通常是通过研究基因产物在细胞中的位置来获得的，DICM共享资源满足了这些需求的一部分。据介绍，该资源已被癌症中心内的30多个实验室使用。

The Digital Imaging and Cell Microinjection (DICM) Shared Resource comprises valuable and modern tools which are made available as routine services to Cancer Center Members. Services include high-resolution digital deconvolution microscopy, digital epi-fluorescence and brightfield microscopy, quantitative image analysis, cell microinjection and subsequent biological analyses, photo-production and training and advice. These services have been crucial for individual lab projects, intra- programmatic research and inter-programmatic research. As research goals and efforts within our Center which address our molecular and cellular functions and intracellular localization of genes implicated in the genesis or growth modulation of cancer cells are emerging with increasing frequency, the Center has taken steps to provide cost-effective support for these cellular and tissue-based studies. This evolution stems from the fact that investigators, such as those in our Cancer Biology and clinical investigation. Programs, have been identifying candidate candidate genes which may be responsible. This has reinforced the fact that the highly important task of solving the functions of these genes is often rate limiting, which is an essential feature of the above programs as well as others in our Center. As clues to the functions of candidate genes are often gained by studying the location of the gene products in cells, the DICM Shared Resource meets a portion of these needs. According, the Resource has been used by over 30 laboratories within the Cancer Center.