Core--Fluorescence microscopy and imaging

荧光显微镜和成像核心（核心 B）将为该计划成员提供荧光显微镜和定量图像处理应用方面的技术专业知识，包括光学切片和三维重建、荧光共振能量转移（FRET）和定量活细胞荧光成像。该核心将位于 UCHC 生物医学成像技术中心 (CBIT)。 CBIT是一家实力雄厚的研究中心，专注于荧光成像技术的开发和应用以及计算模型在细胞生物学研究中的应用。生物医学成像技术中心 (CBIT) 副主任 AnncOWAN 博士将领导该核心项目。核心提供的服务将包括使用最先进的仪器和软件并提供技术援助，以及荧光成像应用的实验设计和数据解释方面的专业知识。核心可用的显微镜​​设施包括 4 台共焦显微镜、2 台具有非线性（多光子）功能的显微镜、2 台配备高灵敏度 CCD 相机的宽场（传统）荧光显微镜工作站，以及大量图像处理、三维渲染和演示软件。

The Fluorescence Microscopy and Imaging Core (Core B) will provide technical expertise in Fluorescence Microscopy and quantitative image processing applications, including optical sectioning and three- dimensional reconstruction, fluorescence resonance energy transfer (FRET) and quantitative live-cell fluorescence imaging, to members of the program. The core will be located at the Center for Biomedical Imaging Technology (CBIT) at UCHC. CBIT is a well-established research center with a focus on the development and application for fluorescence imaging technology and the use of computational modeling in cell biological research. Dr. AnncOWAN, Deputy Director of the Center for Biomedical Imaging Technology (CBIT) will direct the Core. Services provided by the core will include access to and technical assistance with state of the art instrumentation and software, and expertise in experimental design and data interpretation for fluorescence imaging applications. Microscopy facilities available at the core include 4 confocal microscopes, 2 with non-linear (multi-photon) capabilities, 2 widefield (traditional) fluorescence microscope workstations equipped with high sensitivity CCD cameras, and a host of image processing, three-dimensional rendering, and presentation software.