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Fluorescene Microscopy core

荧光显微镜核心

基本信息

批准号：
7662918
负责人：
ANN E. COWAN
金额：
$ 8.9万
依托单位：
UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-26 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7662918
关键词：
Area Arts Atherosclerosis Berlin Biological Assay Biology Blood Vessels Cell Culture Techniques Cell model Cell physiology Cells Cellular biology Charge Collaborations Communities Complex Computer Simulation Computer software Consult Data Development Disease Environment Equipment Event Experimental Designs Fees Floor Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Funding Generations Goals Hand Home environment Hour Human Resources Image Image Analysis Imagery Imaging technology Immunofluorescence Immunologic Individual Instruction Internet Laboratories Life Location Maintenance Maps Measurement Methodology Methods Microinjections Microscope Microscopy Modeling Molecular Molecular Analysis NRCAM gene National Center for Research Resources Operative Surgical Procedures Optics Pathway interactions Philosophy Photobleaching Photons Postdoctoral Fellow Preparation Process Publications Research Research Personnel Research Project Grants Resources Running Services Signal Pathway Signal Transduction Simulate Specimen Spectrum Analysis Students Technical Expertise Techniques Technology Time Training Work base biological research cellular imaging computerized tools design experience experimental analysis fluorescence imaging graduate student image processing instrumentation light microscopy mathematical model member photoactivation programs ratiometric research facility research study second harmonic simulation tool vascular inflammation virtual

项目摘要

The Fluorescence Microscopy Core provides technical expertise in fluorescence microscopy techniques and quantitative image processing applications, including optical sectioning and multi-dimensional imaging, nonlinear optical microscopy (aka 2-photon imaging), fluorescence resonance energy transfer (FRET), total internal reflection fluorescence (TIRF) and quantitative live-cell fluorescence imaging. Services provided by the core include access to and technical assistance with state of the art instrumentation and software, and expertise in experimental design and data interpretation for fluorescence imaging applications. The core is located at the Richard D. Berlin Center for Cell Analysis and Modeling (CCAM; www.ccam.uchc.edu), and is situated on the same floor as the Center for Vascular Biology. The close proximity between CCAM and the Center for Vascular Biology makes for easy and extensive interactions between CCAM and the program group. RELEVANCE (See instructions): The Fluorescence Microscopy Core will assist the PPG investigators with sophisticated experiments that use fluorescence microscopy and related techniques, The aim of the experiments are to visualize cells at very high magnification so that the disease process of atherosclerosis is better understood.

荧光显微镜核心提供荧光显微镜技术和定量图像处理应用，包括光学切片和多维成像，非线性光学显微镜（又名 2 光子成像）、荧光共振能量转移 (FRET)、总计内反射荧光 (TIRF) 和定量活细胞荧光成像。提供的服务核心包括获得最先进的仪器和软件并提供技术援助，以及荧光成像应用的实验设计和数据解释方面的专业知识。核心是位于 Richard D. Berlin 细胞分析和建模中心 (CCAM; www.ccam.uchc.edu)，与血管生物学中心位于同一楼层。 CCAM 与 CCAM 之间的距离很近血管生物学中心使 CCAM 和该计划之间能够轻松、广泛地互动团体。相关性（参见说明）：荧光显微镜核心将协助 PPG 研究人员进行复杂的实验，这些实验使用荧光显微镜和相关技术，实验的目的是在非常高的条件下可视化细胞高放大倍数使动脉粥样硬化的疾病过程得到更好的了解。