Core--Biochemical and Analytical Core

本计划项目资助的生化核心实验室将提供一个统一的、高度专业化的、设备齐全的和专业人员配备的单位，能够根据项目研究人员的要求进行各种放射免疫测定、生化和HPLC分析。可用的测定包括使用HPLC测定的NOS活性测量、Eclase和20-HETE的荧光HPLC测量、AA代谢的HPLC分析、磷脂酶活性和cAMP、cGMP、IP 3和DAG的组织浓度的测量。 集中生化中心机构将维护所有分析设备，确保本项目中不同实验室之间的试验结果一致，并将维护完整的集中记录，并为每次试验提供明确的质量控制指南。将人员和设备集中在生化核心实验室，将使实验室能够以更有效和更具成本效益的方式进行常规生化分析，而不是由研究人员在各自的实验室内进行这些分析。在该中心设施中获得分析专业知识和大量设备对于本计划的每个拟议项目都非常重要，并且在拟议研究中需要进行大量生化和HPLC测定，因此支持我们使用该设施是合理的。

The Biochemical Core Laboratory of this Program Project Grant will provide a consolidated, highly specialized, well equipped and professionally staffed unit capable of performing a variety of radioimmunoassay, biochemical and HPLC based analysis as required by project investigators. The available assays include measurements of NOS activity using a HPLC assay, fluorescent HPLC measurement of EETs and 20-HETE, HPLC analysis of the metabolism of AA, phospholipase activity and measurements of tissue concentrations of cAMP, cGMP, IP3 and DAG. The centralized Biochemical Core facility will maintain all of the analytical equipment, ensure uniformity of assay results between the different laboratories in this program and will maintain complete, centralized records and provide well-defined quality control guidelines for each assay. Centralizing the personnel and equipment in the Biochemical Core laboratory will allow the laboratory to perform routine biochemical analyses in a more efficient and cost-effective manner than if the investigators had setup to perform these analyses within their individual laboratories. Access to the analytical expertise and tremendous amount of equipment in this central facility is very important to each of the proposed projects of this Program and support for our use of this facility is justified by the large number of biochemical and HPLC determinations required in the proposed studies.