A High-Performance Food Pathogen Detection Instrument

高性能食品病原体检测仪器

• 批准号: 6643912
• 负责人: XIAOLI SU
• 金额: $ 10万
• 依托单位: BIODETECTION INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2004-06-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6643912
• 关键词: Campylobacter; Escherichia coli 0157:H7; Listeria; Salmonella typhimurium; antigen antibody reaction; bacteria infection mechanism; bacterial food poisoning; biohazard detection; bioreactors; biosensor device; biotechnology; food contamination; food quality /standard; optics; spectrometry; technology /technique development

DESCRIPTION (provided by applicant): Food safety remains a critical issue for human health in our society. The food system in our nation is vulnerable to contamination from natural pathogens and from bio-terrorist attack. Current detection methods are inadequate due to the time-consuming and laboratory-centered approaches presently in use. Instruments for rapid on-site detection of pathogens in food are urgently needed. The objective of this project is to evaluate an innovative capillary bioseparator / bioreactor-based optical biosensor technology for rapid detection of E. coli O157:H7. The detection limit, specificity and detection time possible with this approach will be evaluated. It is expected that the technology will provide <100 cells/ml detection limit, <1 h detection time, and high specificity to E. coli O157:H7 (<1% false positive/negative data). Although the initial work will focus on food safety, similar technology can be utilized for rapid, on-site screening of human E. coli infections. Rapid screening of human infections is also very important because life-threatening E. coli infections are increasing especially in susceptible groups such as premature babies. Preliminary data indicate that the proposed approach has good potential to significantly improve the state-of-the-art in rapid, sensitive, and specific detection of E. coli O157:H7 and eventually other biological pathogens.

描述(申请人提供)：在我们的社会中，食品安全仍然是人类健康的关键问题。我国的食品系统容易受到自然病原体和生物恐怖袭击的污染。由于目前使用的是耗时的、以实验室为中心的方法，目前的检测方法是不够的。迫切需要现场快速检测食品中病原体的仪器。本项目的目的是评估一种创新的基于毛细管生物分离器/生物反应器的光学生物传感器技术，用于快速检测大肠杆菌O157：H7。将对该方法的检测限度、特异性和检测时间进行评估。预计该技术将提供&lt；100个/毫升的检测下限，&lt；1h的检测时间，以及对大肠杆菌O157：H7的高度特异性(&lt；1%的假阳性/阴性数据)。虽然最初的工作将集中在食品安全上，但类似的技术也可以用于快速、现场筛查人类大肠杆菌感染。人类感染的快速筛查也非常重要，因为威胁生命的大肠杆菌感染正在增加，特别是在早产儿等易感人群中。初步数据表明，建议的方法在快速、灵敏和特异地检测大肠杆菌O157：H7以及最终检测其他生物病原体方面具有良好的潜力。