A Versatile Standard for Quantitative Light Microscopy

定量光学显微镜的通用标准

• 批准号: 6644466
• 负责人: ROBERT Arthur ROSS
• 金额: $ 26.83万
• 依托单位: VIRGINIA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-16 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6644466
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; light emission; light intensity; light microscopy; light scattering; measurement; optics

DESCRIPTION (provided by applicant): Quantitative light microscopy is a valuable technique for studies of cellular function. New data concerning interactions of intracellular proteins, ion concentrations, membrane potential and additional forms of cellular activity are obtained from quantitative measurement of light from exogenous and endogenous optical probes. The accuracy of these measurements relies heavily on well calibrated and characterized microscope light detection systems. This proposal describes the commercial development of three instruments to benefit quantitative microscopy. The first instrument, Benchmark TM, is a versatile standard for quantitative light microscopy. A reliable and easily used high precision standard is not commercially available and would greatly facilitate reproducible measurements. Technologies currently used as light standards for microscopy present some practical difficulties. The calibrated light output of these devices is affected by illuminator lamp noise and aging. The light output of the electronic standard described in this proposal is independent of the microscope's illuminator output. Benchmark TM provides repeatable fiat-field illumination at multiple user selectable wavelengths and illumination levels from a single aperture. The second instrument, ilium TM can be used to monitor and record the intensity level of the microscope's illuminator. The third instrument, RatioLite TM, will provide dual wavelength emission for ratiometric recording of fluorescent dyes. These instruments are uniquely versatile, having operating modes unavailable in any competing product. This technology represents a significant technical innovation in the field of light microscopy.

描述（由申请人提供）：定量光学显微镜是研究细胞功能的一项有价值的技术。从外源和内源光学探针的光定量测量中获得了关于细胞内蛋白质相互作用、离子浓度、膜电位和其他形式的细胞活性的新数据。这些测量的准确性在很大程度上依赖于良好校准和表征显微镜光检测系统。