A Versatile Standard for Quantitative Light Microscopy

定量光学显微镜的通用标准

• 批准号: 6710663
• 负责人: ROBERT Arthur ROSS
• 金额: $ 24.19万
• 依托单位: VIRGINIA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-16 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6710663
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; light emission; light intensity; light microscopy; light scattering; measurement; optics

DESCRIPTION (provided by applicant): Quantitative light microscopy is a valuable technique for studies of cellular function. New data concerning interactions of intracellular proteins, ion concentrations, membrane potential and additional forms of cellular activity are obtained from quantitative measurement of light from exogenous and endogenous optical probes. The accuracy of these measurements relies heavily on well calibrated and characterized microscope light detection systems. This proposal describes the commercial development of three instruments to benefit quantitative microscopy. The first instrument, Benchmark TM, is a versatile standard for quantitative light microscopy. A reliable and easily used high precision standard is not commercially available and would greatly facilitate reproducible measurements. Technologies currently used as light standards for microscopy present some practical difficulties. The calibrated light output of these devices is affected by illuminator lamp noise and aging. The light output of the electronic standard described in this proposal is independent of the microscope's illuminator output. Benchmark TM provides repeatable fiat-field illumination at multiple user selectable wavelengths and illumination levels from a single aperture. The second instrument, ilium TM can be used to monitor and record the intensity level of the microscope's illuminator. The third instrument, RatioLite TM, will provide dual wavelength emission for ratiometric recording of fluorescent dyes. These instruments are uniquely versatile, having operating modes unavailable in any competing product. This technology represents a significant technical innovation in the field of light microscopy.

描述（由申请人提供）：定量光学显微镜是研究细胞功能的一种有价值的技术。关于细胞内蛋白质，离子浓度，膜电位和其他形式的细胞活性的相互作用的新数据获得从定量测量的光从外源性和内源性的光学探针。这些测量的准确性在很大程度上依赖于良好的校准和表征显微镜光检测系统。 该提案描述了三种仪器的商业开发，以利于定量显微镜。第一台仪器Benchmark TM是定量光学显微镜的通用标准。可靠且易于使用的高精度标准品在商业上不可用，并且将极大地促进可重复的测量。目前用作显微镜光标准的技术存在一些实际困难。这些设备的校准光输出受照明灯噪声和老化的影响。本建议书中描述的电子标准的光输出与显微镜的照明器输出无关。基准TM提供可重复的平场照明在多个用户可选的波长和照明水平从一个单一的光圈。第二种仪器ilium TM可用于监测和记录显微镜照明器的强度水平。第三台仪器RatioLite TM将提供双波长发射，用于荧光染料的比率记录。这些仪器具有独特的通用性，具有任何竞争产品都无法提供的操作模式。这项技术是光学显微镜领域的一项重大技术创新。