A VERSATILE STANDARD FOR QUANTITATIVE LIGHT MICROSCOPY

定量光学显微镜的通用标准

• 批准号: 6292130
• 负责人: ROBERT Arthur ROSS
• 金额: $ 9.98万
• 依托单位: VIRGINIA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-16 至 2001-10-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292130
• 关键词: biomedical equipment development; light emission; light intensity; light microscopy; light scattering; optics

Quantitative light microscopy is a valuable technique for studies of cellular function. New data concerning interactions of intracellular proteins, ion concentrations, membrane potential and additional forms of cellular activity are obtained from quantitative measurement of light from exogenous and endogenous optical probes. The accuracy of these measurements relies heavily on well calibrated and characterized microscope light detection systems. This proposal describes the development of a versatile calibration standard for quantitative light microscopy. A reliable and easily used high precision standard is not commercially available and would greatly facilitate reproducible measurements. Technologies currently used as light standards for microscopy present some practical difficulties. The calibrated light output of these devices is affected by illuminator lamp noise and aging. The light output of the electronic standard described in this proposal is independent of the microscope's illuminator output. It provides repeatable flat-field illumination at multiple user selectable wavelengths and illumination levels from a single aperture. Additionally, the instrument can be used to monitor and record the intensity level of the microscope's illuminator. The instrument is uniquely versatile, having operating modes unavailable in any competing product. This technology represents a significant technical innovation in the field of light microscopy. PROPOSED COMMERCIAL APPLICATIONS: The versatile electronic standard described in this proposal represents a new approach for the calibration of light microscopy equipment. It has significant advantages compared to competing technologies, such as neutral density filters or fluorescent beads. Because of these advantages, this technology could capture a significant portion of the $50 million microscope calibration accessory market.

定量光学显微镜是研究细胞功能的一种有价值的技术。关于细胞内蛋白质，离子浓度，膜电位和其他形式的细胞活性的相互作用的新数据获得从定量测量的光从外源性和内源性的光学探针。这些测量的准确性在很大程度上依赖于良好的校准和表征显微镜光检测系统。该建议描述了一个通用的定量光学显微镜校准标准的发展。可靠且易于使用的高精度标准品在商业上不可用，并且将极大地促进可重复的测量。目前用作显微镜光标准的技术存在一些实际困难。这些设备的校准光输出受照明灯噪声和老化的影响。本建议书中描述的电子标准的光输出与显微镜的照明器输出无关。它提供了可重复的平场照明在多个用户可选的波长和照明水平从一个单一的光圈。此外，该仪器可用于监测和记录显微镜照明器的强度水平。该仪器具有独特的多功能性，具有任何竞争产品都无法提供的操作模式。这项技术是光学显微镜领域的一项重大技术创新。拟议的商业应用：本建议书中描述的多功能电子标准代表了光学显微镜设备校准的新方法。与竞争技术相比，它具有显着的优势，例如中性密度过滤器或荧光珠。由于这些优势，这项技术可以占据5000万美元的显微镜校准配件市场的很大一部分。