MAGNETICALLY READABLE MICROARRAY

磁力可读微阵列

• 批准号: 6646178
• 负责人: Val V. Golovlev
• 金额: $ 34.29万
• 依托单位: SCI-TEC, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-11-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6646178
• 关键词: biomedical automation; biomedical equipment development; cell line; chemical synthesis; magnetic field; microarray technology; nucleic acid hybridization; nucleic acid quantitation /detection; polymerase chain reaction; technology /technique development

DESCRIPTION (provided by applicant): The goal of this research project is to develop a new technology for microarray analysis that has superior performance and is more affordable than the currently available fluorescence-based microarrays. In Phase-I we have provided proof-of-principle for the proposed approach of reading a DNA microarray produced directly on the surface of a computer diskette. With this approach, a modified disk drive of a personal computer can be used as a reader device. Potential applications of this new technology can include: (1) Creating new diagnostic classifications based on gene expression; (2) Discovery of signaling pathways and new targets of therapy; (3) Developing new markers of diseases; (4) Monitoring of treatment response and selection of the most appropriate therapy. During Phase II, we will produce a commercial prototype of the Magnetic Array System and, after careful evaluation and validation in Phase-II, we will make this system available for commercial production in Phase III. The objectives in Phase II will be achieved through the specific aims established as following: (1) The Magnetic Array System will be optimized by upgrading Array Reader Electronics; by developing a methodology for automated data filtering and quantitative characterization of the quality of the microarray data; and by introducing 3D-linker technology for producing high-performance microarrays in a format compatible with the magnetic array reader. (2) Microarray processing protocols will be finalized and a pilot production of the focused microarrays will be established for applications in cancer research. (3) The performance of the prototype of the Magnetic Array System will be evaluated through collaboration with research groups in academic institutions. For successful completion of Phase-II, Sci-Tec Inc. has established collaboration with academic institutions and businesses including the Material Analysis Center at Oak Ridge National Laboratory, the Medical Center of the University of Tennessee, the E.O. Lawrence Berkeley National Laboratory, Link Instruments (Fairfield, NJ), Zylux (Oak Ridge, TN), and Southeast Community Capital, a venture capital company organized to help and assist new technology businesses.

描述（由申请人提供）：本研究项目的目标是开发一种新的微阵列分析技术，该技术具有上级性能，并且比目前可用的基于荧光的微阵列更经济实惠。在第一阶段，我们已经为所提出的直接在计算机磁盘表面上产生的阅读DNA微阵列的方法提供了原理证明。通过这种方法，个人计算机的修改的磁盘驱动器可以用作读取器设备。这项新技术的潜在应用包括：（1）根据基因表达建立新的诊断分类;（2）发现信号通路和新的治疗靶点;（3）开发新的疾病标志物;（4）监测治疗反应和选择最合适的治疗方法。 在第二阶段，我们将生产一个磁阵列系统的商业原型，在第二阶段经过仔细的评估和验证后，我们将在第三阶段将该系统投入商业生产。第二阶段的目标将通过以下具体目标实现：（1）将通过升级阵列阅读器电子设备来优化磁阵列系统;通过开发自动数据过滤和定量表征微阵列数据质量的方法;并通过引入3D连接器技术，以与磁阵列阅读器兼容的格式生产高性能微阵列。(2)微阵列处理协议将最后确定，并将建立用于癌症研究的重点微阵列的试点生产。(3)将通过与学术机构的研究小组合作，对磁阵列系统原型的性能进行评估。 为了顺利完成第二阶段，Sci-Tec Inc.与学术机构和企业建立了合作关系，包括橡树岭国家实验室的材料分析中心、田纳西大学医学中心、E.O.劳伦斯伯克利国家实验室，链接仪器（费尔菲尔德，新泽西州），Zylux（橡树岭，田纳西州），和东南社区资本，一个风险投资公司组织，以帮助和协助新技术企业。