BIOELECTRONIC MICROARRAY FOR DNA ANALYSIS

用于 DNA 分析的生物电子微阵列

• 批准号: 6641005
• 负责人: Val V. Golovlev
• 金额: $ 9.95万
• 依托单位: SCI-TEC, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2003-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6641005
• 关键词: DNA; biotechnology; consumable /disposable biomedical equipment; electrochemistry; microarray technology; nucleic acid hybridization; nucleic acid quantitation /detection; oligonucleotides; technology /technique development

In this project, we propose a new detection technology for identification of oligonucleotides, which works by detecting a bio-electronic response from hybridized duplexes. Compared with the conventional approach of using dye tagged oligos, the proposed electrochemical detector has significant advantages. Electrochemical sensing can be implemented using relatively simple equipment. Analysis can be performed in solution. In the proposed approach, no tagging of target molecules is required. When fully developed, highly integrated electronic chips, previously developed for imaging applications, can be modified and can be used as a new generation of inexpensive, disposable, electrochemical DNA microarray sensors.

在这个项目中，我们提出了一种用于识别寡核苷酸的新检测技术，该技术通过检测杂交双链体的生物电子响应来工作。与使用染料标记寡核苷酸的传统方法相比，所提出的电化学检测器具有显着的优势。电化学传感可以使用相对简单的设备来实现。分析可以在溶液中进行。在所提出的方法中，不需要对目标分子进行标记。先前为成像应用而开发的高度集成的电子芯片在完全开发后，可以进行修改并用作新一代廉价、一次性的电化学 DNA 微阵列传感器。