Bioluminscence Assay for Gene Expression Analysis

用于基因表达分析的生物发光测定

基本信息

批准号：
7219640
负责人：
Val V. Golovlev
金额：
$ 10万
依托单位：
SCI-TEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-30 至 2007-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7219640
关键词：
bioassay bioengineering /biomedical engineering bioluminescence clinical research cost effectiveness gene expression profiling messenger RNA method development microRNAs nucleic acid quantitation /detection polymerase chain reaction pyrophosphates

项目摘要

DESCRIPTION (provided by applicant): Project Summary/Abstract: The goal of this project is to develop a new highly sensitive and cost effective Reverse Transcription Quantitative Bioluminescence Assay (RT-qBLA) for high-throughput detection and quantification of large (mRNA) and small (microRNA) RNAs in diverse biological samples. The RT-qBLA implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of RNA molecules for gene expression analysis. The proposed assay can successfully challenge the real time quantitative RT-PCR technique (RT-qPCR) in many applications and can be superior to RT-qPCR particularly for the analysis of small RNA molecules. The RT-qBLA is requiring less expensive reagents and equipment and can be used in a number of commercial assays for applications in life sciences research, drug discovery, clinical diagnosis, environmental analysis, and biodefense. Project Narrative: The goal of this project is to develop a new high performance cost effective bioluminescence assay for gene expression analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis and biodefense in more than 1,800 clinical laboratories and thousands of life science research laboratories in the U.S.

描述（由申请人提供）：项目摘要/摘要：该项目的目的是开发一种新的高度敏感且具有成本效益的逆转录逆转录定量生物发光测定法（RT-QBLA），以在多样性生物学样本中对大通量检测和量化大型（mRNA）和小（mRNA）RNAS的量化。 RT-QBLA实现了从焦磷酸测序已知的相同检测概念，但扩展了焦磷酸测序检测方法，以高度敏感和准确地定量RNA分子进行基因表达分析。所提出的测定可以成功地挑战许多应用中的实时定量RT-PCR技术（RT-QPCR），并且可以优于RT-QPCR，尤其是用于分析小RNA分子。 RT-QBLA需要较便宜的试剂和设备，可用于生命科学研究，药物发现，临床诊断，环境分析和生物化的许多商业测定中。项目叙述：该项目的目的是开发一种用于基因表达分析的新的高性能成本有效生物发光测定。拟议的测定法为实时聚合酶链反应技术提供了技术先进的替代方案，该反应技术目前在市场中占主导地位。拟议的生物发光测定是通用的，可用于生命科学研究，药物发现，临床诊断和生物延展性的各种应用中，在美国超过1,800个临床实验室和数千个生命科学研究实验室中