Bioluminscence Assay for Gene Expression Analysis

项目概述/摘要：本项目的目标是开发一种新的高灵敏度和高成本效益的逆转录定量生物发光测定（RT-qBLA），用于高通量检测和定量各种生物样品中的大（mRNA）和小（microRNA） rna。RT-qBLA实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，用于基因表达分析的RNA分子的高灵敏度和准确定量。所提出的分析方法可以在许多应用中成功挑战实时定量RT-PCR技术（RT-qPCR），并且可以优于RT-qPCR，特别是对于小RNA分子的分析。RT-qBLA需要更便宜的试剂和设备，可用于生命科学研究、药物发现、临床诊断、环境分析和生物防御等许多商业分析。项目描述：本项目的目标是开发一种新的高性能、低成本的基因表达分析生物发光法。拟议的检测方法为实时聚合酶链反应技术提供了技术先进且价格低廉的替代方案，该技术目前在市场上占主导地位。提出的生物发光分析是通用的，可以在美国1800多个临床实验室和数千个生命科学研究实验室中用于生命科学研究、药物发现、临床诊断和生物防御的各种应用

DESCRIPTION (provided by applicant): Project Summary/Abstract: The goal of this project is to develop a new highly sensitive and cost effective Reverse Transcription Quantitative Bioluminescence Assay (RT-qBLA) for high-throughput detection and quantification of large (mRNA) and small (microRNA) RNAs in diverse biological samples. The RT-qBLA implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of RNA molecules for gene expression analysis. The proposed assay can successfully challenge the real time quantitative RT-PCR technique (RT-qPCR) in many applications and can be superior to RT-qPCR particularly for the analysis of small RNA molecules. The RT-qBLA is requiring less expensive reagents and equipment and can be used in a number of commercial assays for applications in life sciences research, drug discovery, clinical diagnosis, environmental analysis, and biodefense. Project Narrative: The goal of this project is to develop a new high performance cost effective bioluminescence assay for gene expression analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis and biodefense in more than 1,800 clinical laboratories and thousands of life science research laboratories in the U.S.