Bioluminscence Assay for Gene Expression Analysis

描述（由申请人提供）：项目摘要/摘要：本项目的目标是开发一种新的高灵敏度和成本效益的逆转录定量生物发光分析（RT-qBLA），用于高通量检测和定量不同生物样品中的大（mRNA）和小（microRNA）RNA。RT-qBLA实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，用于基因表达分析的RNA分子的高灵敏度和准确定量。所提出的测定法可以在许多应用中成功地挑战真实的时间定量RT-PCR技术（RT-qPCR），并且可以上级于RT-qPCR，特别是对于小RNA分子的分析。RT-qBLA需要更便宜的试剂和设备，可用于生命科学研究、药物发现、临床诊断、环境分析和生物防御中的许多商业测定。项目叙述：本项目的目标是开发一种新的高性能、高性价比的生物发光检测方法，用于基因表达分析。所提出的测定法为真实的时间聚合酶链反应技术提供了技术先进且显著更便宜的替代方案，该技术目前在市场上占主导地位。所提出的生物发光测定是通用的，并且可以在美国超过1，800个临床实验室和数千个生命科学研究实验室中用于生命科学研究、药物发现、临床诊断和生物防御中的各种应用。

DESCRIPTION (provided by applicant): Project Summary/Abstract: The goal of this project is to develop a new highly sensitive and cost effective Reverse Transcription Quantitative Bioluminescence Assay (RT-qBLA) for high-throughput detection and quantification of large (mRNA) and small (microRNA) RNAs in diverse biological samples. The RT-qBLA implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of RNA molecules for gene expression analysis. The proposed assay can successfully challenge the real time quantitative RT-PCR technique (RT-qPCR) in many applications and can be superior to RT-qPCR particularly for the analysis of small RNA molecules. The RT-qBLA is requiring less expensive reagents and equipment and can be used in a number of commercial assays for applications in life sciences research, drug discovery, clinical diagnosis, environmental analysis, and biodefense. Project Narrative: The goal of this project is to develop a new high performance cost effective bioluminescence assay for gene expression analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis and biodefense in more than 1,800 clinical laboratories and thousands of life science research laboratories in the U.S.