BIOLUMINESCENT ASSAY FOR GENE EXPRESSION ANALYSIS

描述（由申请人提供）：该项目的目标是开发一种新的高灵敏度和高成本效益的RNA生物发光测定法（RT-qBLA），用于生物样品中RNA和DNA的高通量检测和定量。该分析实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序方法，用于高度敏感和准确的RNA和DNA分子定量。该方法具有独特的灵敏度和动态范围，能够实时测量溶液中RNA和DNA复制的动力学，而不需要实时PCR的长时间和昂贵的样品扩增。RT-qBLA需要比实时PCR和DNA微阵列更便宜的试剂和设备，可用于生命科学研究，药物发现和临床诊断的各种商业分析。

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Bioluminescent Assay (RT-qBLA) for high-throughput detection and quantification of RNA and DNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing methodology for highly sensitive and accurate quantification of RNA and DNA molecules. The proposed assay has unique sensitivity and dynamic range and is able to measure kinetics of RNA and DNA replication in the solution in real time without long and expensive sample amplification of the real time PCR. The RT-qBLA requires less expensive reagents and equipment than real time PCR and DNA microarrays and can be used in a various commercial assays in life sciences research, drug discovery, and clinical diagnosis.