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BIOLUMINESCENT ASSAY FOR GENE EXPRESSION ANALYSIS

用于基因表达分析的生物发光测定

基本信息

批准号：
7617864
负责人：
Val V. Golovlev
金额：
$ 36.44万
依托单位：
SCI-TEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-30 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7617864
关键词：
Antibiotic Resistance Antiviral Agents Area Biochemical Reaction Biological Biological Assay Biological Models Biological Sciences Bioluminescence Buffers Collaborations Complementary DNA Computer software DNA DNA Microarray Chip DNA biosynthesis Detection Development Diphosphates Equipment Foundations Future Gel Gene Expression Genes Genetic Transcription Genomics Genotype Goals HIV-1 Human Individual Kinetics Label Letters Light Literature Liver Luciferases Manufacturer Name Measurement Measures Methodology Mission Monitor National Center for Research Resources National Heart, Lung, and Blood Institute National Human Genome Research Institute National Institute of Diabetes and Digestive and Kidney Diseases National Institute of General Medical Sciences National Institute of Neurological Disorders and Stroke Nucleic Acids Nucleotides Oligonucleotides Performance Pharmaceutical Preparations Phase Photons Polymerase Progress Reports Protocols documentation PubMed Publications Publishing RNA RNA replication Radioactive Reaction Reagent Reporting Research Reverse Transcription Sampling Series Signal Transduction Site Solid Solutions Staphylococcus aureus Sulfate Adenylyltransferase System Techniques Technology Temperature Tennessee Testing Time United States National Institutes of Health Universities Validation Veterinary Medicine Visible Radiation Work base clinical Diagnosis clinical application college commercialization cost cost effective design drug discovery high throughput screening inhibitor/antagonist instrument instrumentation luciferin microbial new technology nucleic acid detection polymerization programs research and development research study technology development

项目摘要

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Bioluminescent Assay (RT-qBLA) for high-throughput detection and quantification of RNA and DNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing methodology for highly sensitive and accurate quantification of RNA and DNA molecules. The proposed assay has unique sensitivity and dynamic range and is able to measure kinetics of RNA and DNA replication in the solution in real time without long and expensive sample amplification of the real time PCR. The RT-qBLA requires less expensive reagents and equipment than real time PCR and DNA microarrays and can be used in a various commercial assays in life sciences research, drug discovery, and clinical diagnosis.

描述（由申请人提供）：本项目的目标是开发一种新的高灵敏度和成本效益的RNA生物发光测定（RT-qBLA），用于生物样品中RNA和DNA的高通量检测和定量。该分析实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序方法，用于RNA和DNA分子的高灵敏度和准确定量。所提出的测定具有独特的灵敏度和动态范围，并且能够在真实的时间内测量溶液中RNA和DNA复制的动力学，而不需要真实的时间PCR的长且昂贵的样品扩增。RT-qBLA需要比真实的时间PCR和DNA微阵列更便宜的试剂和设备，并且可以用于生命科学研究、药物发现和临床诊断中的各种商业测定。