BIOCHEMISTRY CORE

生物化学核心

• 批准号: 6480655
• 负责人: ARI GAFNI
• 金额: $ 8.33万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480655
• 关键词: adenosine triphosphate; aging; biochemistry; biomedical facility; computer data analysis; cytoskeletal proteins; data collection methodology /evaluation; data management; glutathione peroxidase; mitochondria; oxidative stress; striated muscles; superoxide dismutase; tissue /cell preparation

The central goal of the biochemistry core is to provide Program researchers with the facilities, instrumentation and expertise in biochemical data collection, handling and analysis that are needed for their research work. The core will serve as a centralized facility for biochemical data collection and processing, will help minimize duplication of effort, equipment and materials, and will provide Program researchers with a cost effective, convenient and high quality service. The specific aims of this core are: Aim 1. To provide Program affiliated researchers with a dedicated, central, facility that they can utilize to perform experiments and biochemical assays that require the use of fresh tissue and therefore need to be done at the University of Michigan. The core will house and maintain all the needed equipment and reagents needed by Program researchers and will provide technical support and training to Program investigators in performing biochemical assays and in analyzing their results. Core personnel will adapt and implement effective methodologies for all biochemical assays needed by Program researchers will train users in applying protocols that they prefer to perform themselves, and will provide them with all needed assistance. Aim 2. To serve Program researchers by performing the biochemical assays needed for the development of their individual projects and by preparing and providing samples to Program researchers to be studied in their laboratories. A large number of assays will be done in the core and their performance in a designated core facility will ensure reproducibility of experimental protocols and conditions and will eliminated duplication of both equipment and manpower. In addition the biochemistry core will serve as a central facility for the preparation of samples to be distributed to Program researchers' laboratories. Aim 3. To achieve two complementary, but not redundant goals: a. to utilize the core's facilities and expertise as a resource that Program investigators can rely on when they need to introduce new biochemical tools into their research and b. to develop new innovative methodologies for biomolecular studies, with enhanced sensitivities and resolving power, and to make them available to Program researchers for their studies. Core personnel will either adapt existing methodologies or develop new approaches on-site to meet specific research needs, and make them available to Program researchers, thereby enhancing their capability to embark on new research directions.

生物化学核心的中心目标是为计划研究人员提供研究工作所需的生化数据收集，处理和分析方面的设施，仪器和专业知识。该核心将作为生化数据收集和处理的集中设施，将有助于最大限度地减少工作、设备和材料的重复，并将为项目研究人员提供具有成本效益、方便和高质量的服务。该核心的具体目标是： 目标1.为项目附属研究人员提供一个专用的中央设施，他们可以利用该设施进行实验和生化分析，这些实验和生化分析需要使用新鲜组织，因此需要在密歇根大学进行。核心将容纳和维护项目研究人员所需的所有设备和试剂，并为项目研究人员提供进行生化测定和分析结果的技术支持和培训。核心人员将调整和实施项目研究人员所需的所有生化分析的有效方法，培训用户应用他们喜欢自己执行的方案，并为他们提供所有必要的帮助。 目标二。为项目研究人员提供项目开发所需的生化分析，并为项目研究人员准备和提供样品，供他们在实验室进行研究。将在堆芯中进行大量测定，在指定的堆芯设施中进行测定将确保实验方案和条件的再现性，并消除设备和人力的重复。此外，生物化学核心将作为制备样本的中央设施，分发给项目研究人员的实验室。 目标3。 实现两个互补但不冗余的目标：a.利用核心的设施和专业知识作为项目研究人员在需要将新的生物化学工具引入其研究时可以依赖的资源，以及B.为生物分子研究开发新的创新方法，提高灵敏度和分辨率，并使其可用于计划研究人员的研究。核心人员将调整现有的方法或现场开发新的方法，以满足特定的研究需求，并将其提供给计划研究人员，从而提高他们开展新研究方向的能力。