Oxidative damage and neural signaling in aging

描述（由申请人提供）：氧化应激可能改变老年大脑中的神经元功能，并通过改变神经元内和神经元之间的通信而导致与年龄相关的认知衰退。了解认知老化的神经表现可能会导致潜在的药物治疗，旨在减轻与年龄相关的认知能力下降。这项拟议中的研究将直接检查老年大脑中专门参与神经信号传导的蛋白质的氧化状态。具体而言，在大鼠和人类中由于衰老而改变的第二信使系统是磷酸肌醇（PI）周转。在老年大鼠中，毒蕈碱和代谢型谷氨酸受体刺激PI营业额在海马中是钝化的，并且在有记录的记忆障碍的老年大鼠中最钝化。在这些相同的大鼠中，海马蛋白的氧化损伤也已被证明，但这些蛋白的身份尚未被指定。拟议的实验将试图确定PI营业额功能障碍的分子位点，这是与认知老化的功能和氧化状态的两个信号蛋白耦合到PI营业额，Gq/11和PLC-β 1。该项目使用已评估空间学习能力的年轻和老年大鼠的海马组织，将解决以下具体目标：1）Gq/11和PLC-beta1是否保持刺激认知障碍老年大鼠海马中PI转换的能力？2)海马、纹状体和额叶皮层中的毒蕈碱型和代谢型谷氨酸受体偶联是否随年龄而改变？3)老年大鼠海马中Gq/11和PLC-β 1是否受到氧化损伤，这种损伤是否与认知障碍相关？4)通过实验诱导体外年轻动物海马中的氧化应激，可以复制Gq/11和PLC-β 1发生的年龄相关的变化吗？如果是这样，该模型随后将用于测试抗氧化剂预防Gq/11和/或PLC-β 1功能氧化损伤的功效。这些研究有可能显示与年龄相关的PI功能障碍的分子位点，功能障碍的原因，以及特定蛋白质的氧化损伤和认知障碍之间的联系。

DESCRIPTION (provided by applicant): Oxidative stress may alter neuron function in the aged brain and contribute to age-related cognitive decline by altering communication within and between neurons. Understanding the neural manifestation of cognitive aging may lead to potential pharmacological therapies intended to attenuate age-related cognitive decline. The proposed research will directly examine the oxidative status of proteins specifically involved in neural signaling in the aged brain. Specifically, a second messenger system that is altered as a consequence of aging in both rats and humans is phosphoinositide (Pl) turnover. In aged rats, muscarinic and metabotropic glutamate receptor stimulation of PI turnover is blunted in the hippocampus, and is most blunted in aged rats with documented memory impairment. In these same rats, oxidative damage to hippocampal proteins has also been demonstrated, but the identity of these proteins has not been specified. The proposed experiments will attempt to identify the molecular site of PI turnover dysfunction that is associated with cognitive aging by examining the function and oxidative status of two signaling proteins coupled to PI turnover, Gq/11 and PLC-beta1. Using hippocampal tissue from young and aged rats that have been assessed for spatial learning ability, this project will address the following specific aims: 1) Do Gq/11 and PLC-beta1 maintain their ability to stimulate PI turnover in the hippocampus of aged rats with cognitive impairment? 2) Is muscarinic and metabotropic glutamate receptor coupling altered with age in the hippocampus, striatum and frontal cortex? 3) Are Gq/11 and PLC-beta1 oxidatively damaged in the hippocampus of the aged rat and does this damage correlate with cognitive impairment? 4) Can the age-related alterations that occur to Gq/11 and PLC-beta1 be replicated by experimentally inducing oxidative stress in the hippocampus of a young animal in vitro? If so, this model will subsequently be used to test the efficacy of antioxidants to prevent oxidative damage to Gq/11 and/or PLC-beta1 function. These studies have the potential to show the molecular site of age-related PI dysfunction, the cause of the dysfunction, and a linkage between oxidative damage to specific proteins and cognitive impairment.