CORE--MORPHOLOGY/MORPHOMETRY

改编自申请者？S摘要)这一核心将提供必要的 肌肉的显微和形态计量学研究的技术设备 其他纸巾。在这个PPG的25年里，调查人员已经 始终坚持结构应与功能相关联的立场。 在方案项目的前两个周期中，广泛使用了 肺快速冷冻结合组织制备技术 在马兹宗博士的指导下进行电子显微镜检查。什么时候 15年前，马佐内医生离开，奥迪勒·马蒂厄-科斯特洛医生接替他成为一名 教职员工。她的主要兴趣是结构-功能关系， 尤其是在骨骼肌中，正如她在第四周期的项目3所证明的那样， 以及上一个周期和本提案中的项目2。她也有 在过去的9年里与约翰·韦斯特博士在电子领域密切合作 肺毛细血管应力衰竭的显微镜和形态计量学研究。她 在两个肺的主要权威之一的培训下成为了一名形态学家 和肌肉形态计量学，埃瓦尔德·韦贝尔教授来自 瑞士。她为这个高度专业化的领域带来了独特的专业知识， 她是继续领导这方面科学研究的理想人选 核心。研究人员将形态和形态测量核心视为 计划项目的基本特征。 核心也将全力支持组织学李伍的工资 技术人员，与上一个周期相同。吴先生在准备方面有经验 肌肉和其他组织的切片进行光学和电子显微镜检查，以及 组织化学。他将与马蒂厄-科斯特洛博士在 光镜和电子显微镜组织的制备，吴先生 很好地理解我们团队的需求，并在执行方面接受过良好的培训 组织准备和形态计量学。形态计量分析需要较大的 要检查的切片和区域的数量，以及组织切片， 尤其是对于电子显微镜来说，是非常耗时的。基于 过去几个周期的核心产出和本年度拟开展的研究 应用程序，显然需要一名全职技术人员来满足 不同的项目。

Adapted from the Applicant?s Abstract) This Core will provide essential technical facilities for microscopic and morphometric studies of muscle and other tissues. Throughout the 25 years of this PPG, the investigators have always taken the stance that structure should be correlated with function. During the first two cycles of the Program Project, extensive use was made of the technique of rapid freezing of lung combined with preparation of tissue for transmission electron microscopy under the direction of Dr. Mazzone. When Dr. Mazzone left, 15 years ago, Dr. Odile Mathieu-Costello replaced him as a faculty member. Her primary interest is in structure-function relationships, especially in skeletal muscle as evidenced by her Project 3 in the 4th cycle , and Project 2 in the previous cycle and in this proposal. She has also closely collaborated with Dr. John West over the past 9 years on the electron microscopy and morphometry of stress failure of pulmonary capillaries. She was trained as a morphologist by one of the leading authorities in both lung and muscle morphology and morphometry, Professor Ewald Weibel from Switzerland. She brings unique expertise to this highly specialized area, and she is the ideal person to continue to head the scientific aspects of this Core. The investigators see the Morphology and Morphometry Core as an essential feature of the Program Project. The Core will also fully support the salary of Li Wu, the histology technician, as in the previous cycle. Mr. Wu has experience in the preparation of sections of muscle and other tissues for light and electron microscopy, and histochemistry. He will work closely with Dr. Mathieu-Costello in the preparation of tissues for both light and electron microscopy, Mr. Wu understands the needs of our group well, and is well trained in the execution of tissue preparation and morphometry. Morphometric analysis requires a large number of sections and fields to be examined, and tissue sectioning, especially for electron microscopy, is extremely time consuming. Based on the output of the Core in the past cycles and the studies proposed in this application, a full time technician is clearly needed to meet the needs of the different projects.