CORE--MORPHOLOGY/MORPHOMETRY

核心--形态学/形态测量学

• 批准号: 6589831
• 负责人: ODILE A MATHIEU-COSTELLO
• 金额: $ 24.62万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6589831
• 关键词: biomedical facility; histology; microscopy; morphometry

Adapted from the Applicant?s Abstract) This Core will provide essential technical facilities for microscopic and morphometric studies of muscle and other tissues. Throughout the 25 years of this PPG, the investigators have always taken the stance that structure should be correlated with function. During the first two cycles of the Program Project, extensive use was made of the technique of rapid freezing of lung combined with preparation of tissue for transmission electron microscopy under the direction of Dr. Mazzone. When Dr. Mazzone left, 15 years ago, Dr. Odile Mathieu-Costello replaced him as a faculty member. Her primary interest is in structure-function relationships, especially in skeletal muscle as evidenced by her Project 3 in the 4th cycle , and Project 2 in the previous cycle and in this proposal. She has also closely collaborated with Dr. John West over the past 9 years on the electron microscopy and morphometry of stress failure of pulmonary capillaries. She was trained as a morphologist by one of the leading authorities in both lung and muscle morphology and morphometry, Professor Ewald Weibel from Switzerland. She brings unique expertise to this highly specialized area, and she is the ideal person to continue to head the scientific aspects of this Core. The investigators see the Morphology and Morphometry Core as an essential feature of the Program Project. The Core will also fully support the salary of Li Wu, the histology technician, as in the previous cycle. Mr. Wu has experience in the preparation of sections of muscle and other tissues for light and electron microscopy, and histochemistry. He will work closely with Dr. Mathieu-Costello in the preparation of tissues for both light and electron microscopy, Mr. Wu understands the needs of our group well, and is well trained in the execution of tissue preparation and morphometry. Morphometric analysis requires a large number of sections and fields to be examined, and tissue sectioning, especially for electron microscopy, is extremely time consuming. Based on the output of the Core in the past cycles and the studies proposed in this application, a full time technician is clearly needed to meet the needs of the different projects.

是否改编自申请人？摘要）这一核心将提供必要的 用于肌肉显微镜和形态测量研究的技术设施， 其他组织。在PPG的25年中，研究人员 他总是认为结构应该与功能相关联。 在方案项目的前两个周期，广泛使用了 肺组织快速冷冻技术 进行透射电子显微镜检查当 博士Mazzone离开了，15年前，Odile Mathieu-Costello博士取代了他， 教员。她的主要兴趣是结构-功能关系， 特别是在骨骼肌中，如她在第四周期的项目3所证明的， 和项目2的报告。她还 在过去的9年里，我与约翰·韦斯特博士密切合作， 肺毛细血管应力衰竭的显微镜和形态计量学。她 作为一名形态学家，我接受了两个肺领域的主要权威之一的培训。 以及肌肉形态学和形态测量学， Switzerland.她为这个高度专业化的领域带来了独特的专业知识， 她是继续领导科学方面的理想人选， 核心研究人员将形态学和形态测量学核心视为 项目的基本特征。 核心也将全力支持李武的薪资，组织学 技术人员，如前一个周期。吴先生有准备的经验 用于光学和电子显微镜检查的肌肉和其他组织切片，以及 组织化学他将与马蒂厄-科斯特洛博士密切合作， 用于光学和电子显微镜检查的组织制备，吴先生 很好地理解了我们团队的需求，并在执行方面受过良好的培训。 组织制备和形态测量的方法。形态分析需要大量的 要检查的切片和视野的数量，以及组织切片， 特别是对于电子显微镜来说，是非常耗时的。基于 在过去的周期中核心的产出和本报告中建议的研究 应用程序，全职技术人员显然需要满足的需求， 不同的项目。