Histology, Tissue Processing and High Content Microscopy

摘要 组织学、组织加工和高含量显微镜核心 组织学、组织处理和高含量显微镜核心将继续服务于 加州大学圣迭戈分校的视力研究人员通过提供专门的组织处理设备和兼职 在眼组织学和免疫组织化学方面有丰富经验的组织技师。组织学、组织加工 高含量的显微镜核心支持石蜡切片和冰冻切片处理以及各种染色 战略。该核心还为视觉研究人员提供1)视线选择建议，具体取决于 研究目的：2)研究样品的石蜡和冷冻封堵；3)石蜡或冷冻组织学切片的制备 通过定制的染色，4)协助对该地区进行适当的解释和精确的显微采样 用于电子显微镜评估和5)高含量共聚焦成像用于药物筛选和 组织标本成像。

ABSTRACT Histology, Tissue Processing and High Content Microscopy Core The Histology, Tissue Processing and High Content Microscopy Core will continue to serve the needs of UCSD vision researchers by providing dedicated tissue processing equipment and a part-time histotechnician experienced in eye histology and immunohistochemistry. The Histology, Tissue Processing and High Content Microscopy Core supports both paraffin and cryosection processing and various staining strategies. The core also provides vision researchers with 1) advice for fixation selection depending on study purpose; 2) paraffin and cryo blocking of study samples; 3) paraffin or cryo histology slide preparation with customized staining, 4) assistance for proper interpretation and precision microsampling of the region of interest for electron microscopy evaluation and 5) high content confocal imaging for drug screening and histologic specimen imaging.