CORE--ANALYTICAL FACILITY /LIPIDS

核心--分析设施/脂质

• 批准号: 6564196
• 负责人: Larry L. Swift
• 金额: $ 11.77万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564196
• 关键词: aminoacid metabolism; analytical chemistry; biomedical facility; clinical research; dietary lipid; dietary trace element; human subject; lipid metabolism; lipid peroxides; nutrition; nutrition related tag; peroxidation; phospholipids; vitamins

DESCRIPTION (adapted from the application): When the Clinical Nutrition Research Unit (CNRU) was first funded in 1979, the only core facility other than the Administrative/Clinical Core was the Analytical Core. The primary function of this core was to develop and validate vitamin-trace mineral assays for the CNRU investigators. With the continuation of the grant in 1984, the Analytical Core was expanded to include amino acid analyses as well as assays for several blood proteins, including retinol binding protein, transferrin, prealbumin, and albumin. In addition, during this period the laboratory developed more sensitive HPLC assays for vitamins, including assays for vitamins A and E, as well as for the vitamers of vitamins B2 and B6. New instrumentation was also added for quantitative amino acid determinations. In addition, because of an increased interest in research involving lipids and lipid peroxidation, we began the process of expanding the laboratory to include quantitative measurements of the lipid components of tissue, cells, and blood. New methods for studying lipid peroxidative injury were also added to the services provided by the core. A growing need for investigations involving amino acid metabolism prompted a stronger collaboration of the Core Laboratory with the Departments of Surgery, Molecular Physiology and Biophysics, and Medicine, in particular the Diabetes Research and Training Center (DRTC). To accommodate all of the needs, the Analytical Core was reorganized once again, and Dr. Larry L. Swift, Department of Pathology, was appointed the Director. The core was subdivided into three areas of specialization: Amino Acid Section, The Lipids/Lipid Peroxidation Section, and the Vitamin/Trace Element Section, with each section directly independently.

描述（改编自应用程序）：当临床营养 研究单位（CNRU）于1979年首次获得资助，是唯一的核心设施， 管理/临床核心是分析核心。主 该核心的功能是开发和验证维生素微量矿物质测定 CNRU的调查员随着1984年补助金的继续， 分析核心扩展到包括氨基酸分析以及测定 对于几种血液蛋白，包括视黄醇结合蛋白，转铁蛋白， 前白蛋白和白蛋白。在此期间，实验室 开发了更灵敏的维生素HPLC测定法，包括 维生素A和E，以及维生素B2和B6的维生素。新 还增加了用于定量氨基酸测定的仪器。在 此外，由于对涉及脂质和 脂质过氧化，我们开始扩大实验室的过程，包括 组织、细胞和血液的脂质成分的定量测量。 研究脂质过氧化损伤的新方法也被添加到 核心提供的服务。越来越多的调查需要涉及 氨基酸代谢促使核心实验室加强合作 与外科、分子生理学和生物物理学系， 糖尿病研究和培训中心（DRTC）。到 为了满足所有需求，分析核心再次重组， Larry L.博士病理学系的斯威夫特被任命为主任。 核心被细分为三个专业领域：氨基酸部分， 脂质/脂质过氧化部分和维生素/微量元素部分， 每个部分都是独立的。