Yeast as a model system for studying Parkinson's Disease

酵母作为研究帕金森病的模型系统

• 批准号: 6640673
• 负责人: Susan L. Lindquist
• 金额: $ 15.9万
• 依托单位: WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-15 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6640673
• 关键词: Parkinson's disease; Saccharomyces cerevisiae; alpha synuclein; cyclic peptides; fluorescence resonance energy transfer; fungal genetics; gene expression; gene mutation; genetic models; green fluorescent proteins; neural degeneration; oxidative phosphorylation; oxidative stress; pathologic process; phospholipase D; protein localization; protein protein interaction; tissue /cell culture; toxicant screening

DESCRIPTION (provided by applicant) Our limited knowledge of the mechanisms underlying PD demands the clevelopment of innovative avenues of investigation. The extensive characterization of the budding yeast Saccharomyces cerevisiae and the many tools developed for its manipulation makes it a powerful system for studying complex biological problems. Alpha-synuclein (aS), one of the established key players in PD, will be the focus of our Study. Preliminary data suggest that many of the basic features of aS biology in mammalian neurons is recapitulated in yeast. The proposed studies should generate a wealth of pilot information pertaining both to normal aS biology and potential therapeutic strategies to synergize with studies in other systems. We will combine our own expertise in yeast genetics and cell biology with the expertise of a group of investigators, ranging from biochemists, to geneticists and neurobiologists.

描述(由申请人提供) 我们对帕金森病潜在机制的有限知识需要分裂 创新的调查途径。对电影的广泛刻画 芽殖酵母酿酒酵母及其开发的多种工具 操控使其成为研究复杂生物学的强大系统 有问题。阿尔法-突触核蛋白(AS)，PD中公认的关键角色之一，将 是我们研究的重点。初步数据显示，许多基本的 哺乳动物神经元中AS的生物学特征在酵母中得到了概括。这个 拟议的研究应产生大量关于以下两方面的试点信息 恢复正常作为生物学和潜在的治疗策略来协同 在其他系统中的研究。我们将结合我们自己在酵母遗传学方面的专业知识 和细胞生物学，拥有一组研究人员的专业知识，范围从 从生物化学家到遗传学家和神经生物学家。