NanoString Instrumentation

描述(申请人提供)：怀特黑德研究所要求以23.5万美元从纳米串技术公司购买ncount“系统。ncount”系统是一种新的基因表达分析方法，提供实时聚合酶链式反应的数据质量，具有更高的多路复用率、更好的吞吐量和更低的成本。NCounter“系统将填补高通量基因表达项目的空白，需要在数百到数千个样本中进行中等范围的多路复用(50-500个基因)。实时PCR或微阵列等传统基因表达技术无法很好地满足这种吞吐量范围。该系统独特的检测技术还激发了对传统RT-PCR和微阵列技术不足的新应用的建议。NCounter”系统将被整合到怀特黑德研究所的基因组技术核心(GTC)的技术组合中。GTC为怀特黑德研究所的20个实验室和世界各地的数十个实验室提供服务，并在成功支持共享仪器作为收费基因组核心设施方面有着悠久的历史。NCounter系统将补充现有的基于微阵列和定量PCR的基因表达分析平台，为各种规模的表达分析项目提供完整的解决方案。 项目描述：测量细胞中基因表达水平的能力是当今几乎每个生物医学研究项目的关键组成部分。传统的基因表达分析技术价格昂贵、耗时长，并受到各种技术限制。 NanoStringnCounter(Tm)是一项革命性的技术，它将为高通量表达分析提供更便宜、更快和技术上更优越的选择，从而使许多研究项目成为可能。

DESCRIPTION (provided by applicant): The Whitehead Institute requests $235,000 for the purchase of an nCounter" system from NanoString Technologies. The nCounter" system is a new approach to gene expression analysis that offers the data quality of Real-Time PCR with higher multiplexing, better throughput and lower cost. The nCounter" system will fill a niche for high throughput gene expression projects requiring mid-range multiplexing (50-500 hundred genes) in hundreds up to thousands of samples. This throughput range is not well served by traditional gene expression technologies like Real-Time PCR or microarrays. The system's unique detection technology also inspires proposals for novel applications where traditional technologies of RT-PCR and microarrays are inadequate. The nCounter" system will be integrated into the technology portfolio of the Whitehead Institute's Genome Technology Core (GTC). The GTC serves 20 labs at the Whitehead Institute and dozens more around the world and has a long history of successfully supporting shared instrumentation as a fee-for-service genome core facility. The nCounter system will complement existing gene expression analysis platforms based on microarrays and quantitative PCR to provide complete solutions for expression analysis projects of all sizes. PROJECT NARRATIVE: The ability to measure levels of gene expression in cells is a critical component of nearly every biomedical research project today. Traditional technologies for gene expression analysis are expensive, time consuming and suffer from a variety of technical limitations. The nCounter(tm) from NanoString is a revolutionary technology that will enable many research projects by providing a cheaper, faster and technically superior option for high throughput expression analysis.