Harnessing the genetic potential for arbuscular mycorrhizal (AM) symbiosis in rice

利用水稻丛枝菌根 (AM) 共生的遗传潜力

• 批准号: 2118594
• 金额: --
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2118594
• 关键词: Harnessing; genetic; potential; arbuscular; mycorrhizal

The broad aim of the PhD is to harness the genetic potential for arbuscular mycorrhizal (AM) symbiosis in rice. Recent establishment of the high coverage, 3000 Oryza sativa (rice) genome sequence dataset through international effort at the International Rice Research Institute (IRRI), Philippines, provides a unique opportunity to investigate genetic diversity in AM symbiosis. In addition, identification of a novel shoot metabolite marker of AM fungi-association- blumenols- allows for development of a high-throughput screening method to interrogate variation in AM outcome between accessions. This provides a strong basis for a Genome Wide Association Study (GWAS) aiming to identify candidate gene variants required for AM symbiosis in rice.The project aim requires attention to the following objectives: 1. To develop a precise, robust protocol to give consistent, blumenol-derived AM colonisation phenotypes on infection of genetically and physiologically variable rice accessions with Rhizophagus irregularis. 2. To perform a GWAS at the International Rice Research Institute aiming to identify candidate gene variants required for AM fungal associations. 3. To characterise and validate the functional and ecological relevance of the identified gene variants. The project will be co-supervised by Dr. Uta Paszkowski, Department of Plant Sciences, University of Cambridge and Dr. Amelia Henry, International Rice Research Institute (IRRI), Philippines. It will involve a first year at the University of Cambridge to establish a reliable protocol for characterisation of colonisation of diverse rice cultivars by R. irregularis. Variation can often be observed in progression and establishment of AM colonisation so this will ensure that the marker phenotype is consistent. In addition, the robustness of the 'blumenol detection' method for detecting variation in AM colonisation in rice plants will be confirmed through collaboration with Prof. Ian Baldwin at the Max Planck Institute for Chemical Ecology, Jena, Germany. The established protocol will then be taken to IRRI, Philippines to conduct the genetic screen during the second year. The final years will then be back at the University of Cambridge to characterise and validate gene variants identified.The proposed studies will deliver donor materials, QTLs and associated marker systems for introgression of QTLs into upland breeding programs. Thus through use of marker-assisted-backcrossing approaches they will readily translate to products, which, once disseminated through IRRI's network with national partners, will have direct impact in upland farmers' fields, and will be evaluated for their utility in wider water-saving rice production systems.

博士学位的广泛目的是利用水稻中杂菌菌根（AM）共生的遗传潜力。最近在菲律宾国际水稻研究所（IRRI）的国际努力中建立了3000个Oryza sativa（大米）基因组序列数据集，这为研究AM共生的遗传多样性提供了独特的机会。此外，鉴定出AM真菌缔合的新型芽代谢产物标记 - blumenols-可以开发高通量筛选方法，以询问辅助之间AM的变化。这为大米中AM共生所需的候选基因变异型（GWAS）提供了强大的基础。该项目的目标需要注意以下目标：1。开发一种准确的，可靠的方案，以使属于遗传和生理的水稻的感染对遗传性变异的rhizeptions感染一致的，blumenol衍生的AM殖民化表型。 2。在国际水稻研究所（International Rice Research Institute）进行GWAS，以确定AM真菌协会所需的候选基因变体。 3。表征和验证已识别基因变异的功能和生态相关性。该项目将由剑桥大学植物科学系UTA Paszkowski博士和菲律宾国际稻米研究所（IRRI）的Amelia Henry博士共同监督。它将涉及剑桥大学的第一年，以建立一项可靠的方案，用于表征R. iNROGRANGULAIS的多种水稻品种殖民化。通常可以在AM定植的进展和建立中观察到变化，因此这将确保标记表型是一致的。此外，将通过与德国耶拿（Jena）Max Planck化学生态学研究所的Max Planck化学生态研究所的Ian Baldwin教授合作来确认“蓝木醇检测”方法检测水稻植物中AM定植变化的鲁棒性。然后，已建立的方案将被带到菲律宾IRRI，在第二年进行遗传筛查。然后，最后几年将回到剑桥大学，以表征和验证所鉴定的基因变异。拟议的研究将提供供体材料，QTL和相关标记系统，以将QTL渗入高地育种计划。因此，通过使用标记辅助的方法，他们将很容易转化为产品，一旦通过Irri与国家合作伙伴的网络传播，该产品将直接影响到高地农民的领域，并将对其在储蓄更广泛的水稻生产系统中的实用性进行评估。