Function of CMF1 Protein During Avian Myogenesis

CMF1 蛋白在禽类肌肉生成过程中的功能

• 批准号: 6666923
• 负责人: MARY Ellen DEES
• 金额: $ 12.47万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6666923
• 关键词: cell cycle proteins; cell differentiation; chick embryo; developmental genetics; gene expression; genetic library; molecular biology information system; molecular cloning; muscle cells; muscle proteins; myoblasts; myogenesis; protein localization; protein structure function; tissue /cell culture; vertebrate embryology

DESCRIPTION (provided by applicant): The candidate is a Pediatric Cardiologist with a research interest in mechanisms of heart development. The application is submitted with the intent to provide mentored time for the candidate to pursue formal graduate level training in developmental biology and to accomplish the proposed research. Accomplishment of these goals will set the groundwork for establishing an Independent research laboratory. This proposal focuses on CMF1, a member of a novel family of proteins involved in the regulation of call division and differentiation during development. CMF1 is unique in its restriction to developing cardiac and skeletal muscle in the developing chick embryo. Its expression precedes that of contractile proteins in the developing heart tube and in skeletal myoblasts in vitro. Importantly, disruption of CMF1 expression disrupts myogenesis. There is evidence that this protein can localize both to the nucleus and to the cytoplasm of the myocyte, and that it may be cleaved Into two functional components. The experiments proposed will determine the patterns of subcellular localization and function of CMF1 proteins. Techniques proposed include generation of antibodies to various regions of the CMF1 protein, functional analyses of putative nuclear localization sequences, identification of CMF1 associated proteins, and in vitro and in vivo disruption of CMF1 functional domains. There is evidence for other, as yet undiscovered, relatives of CMF1 In mouse and possibly humans. Novel approaches to identify CMF1 related proteins are Included in this proposal. The overall goal is to elucidate novel mechanisms that regulate myogenesis and of developmental processes in general.

描述（由申请人提供）：