Function of CMF1 Protein During Avian Myogenesis
CMF1 蛋白在禽类肌肉生成过程中的功能
基本信息
- 批准号:7104973
- 负责人:
- 金额:$ 12.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-09-30 至 2007-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (provided by applicant):
The candidate is a Pediatric Cardiologist with a research interest in
mechanisms of heart development. The application is submitted with the intent
to provide mentored time for the candidate to pursue formal graduate level
training in developmental biology and to accomplish the proposed research.
Accomplishment of these goals will set the groundwork for establishing an
Independent research laboratory.
This proposal focuses on CMF1, a member of a novel family of proteins involved
in the regulation of call division and differentiation during development.
CMF1 is unique in its restriction to developing cardiac and skeletal muscle
in the developing chick embryo. Its expression precedes that of contractile
proteins in the developing heart tube and in skeletal myoblasts in vitro.
Importantly, disruption of CMF1 expression disrupts myogenesis. There is
evidence that this protein can localize both to the nucleus and to the
cytoplasm of the myocyte, and that it may be cleaved Into two functional
components. The experiments proposed will determine the patterns of
subcellular localization and function of CMF1 proteins. Techniques proposed
include generation of antibodies to various regions of the CMF1 protein,
functional analyses of putative nuclear localization sequences, identification
of CMF1 associated proteins, and in vitro and in vivo disruption of CMF1
functional domains. There is evidence for other, as yet undiscovered,
relatives of CMF1 In mouse and possibly humans. Novel approaches to identify
CMF1 related proteins are Included in this proposal.
The overall goal is to elucidate novel mechanisms that regulate myogenesis and
of developmental processes in general.
描述(由申请人提供):
候选人是一名儿科心脏病专家,研究兴趣为
心脏发育的机制。提交申请的目的是
为候选人提供指导时间,以追求正式的研究生水平
在发育生物学方面的培训,并完成拟议的研究。
实现这些目标将为建立一个
独立的研究实验室。
这项建议的重点是CMF1,一个新的蛋白质家族的成员,
在发育过程中对叫声的划分和分化的调节。
CMF1在限制心肌和骨骼肌发育方面是独特的
在发育中的鸡胚中。它的表达先于收缩的表达,
在体外发育的心管和骨骼肌成肌细胞中的蛋白质。
重要的是,CMF1表达的破坏会破坏肌生成。有
证据表明,这种蛋白质可以定位于细胞核和细胞外基质。
细胞质,它可以被切割成两个功能性的细胞,
件.所提出的实验将确定
CMF1蛋白的亚细胞定位和功能。提出的技术
包括产生针对CMF1蛋白的不同区域的抗体,
推定的核定位序列的功能分析,鉴定
CMF1相关蛋白的表达,以及CMF1在体外和体内的破坏
功能域有证据表明,其他尚未发现的,
CMF1在小鼠和可能的人类中的亲戚。新的方法来识别
CMF1相关蛋白也包括在本提案中。
总体目标是阐明调节肌生成的新机制,
一般的发展过程。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('MARY Ellen DEES', 18)}}的其他基金
Role of LEK and Rb Protein Interactions in Heart and Skeletal Muscle Development
LEK 和 Rb 蛋白相互作用在心脏和骨骼肌发育中的作用
- 批准号:
8271375 - 财政年份:2009
- 资助金额:
$ 12.47万 - 项目类别:
Role of LEK and Rb Protein Interactions in Heart and Skeletal Muscle Development
LEK 和 Rb 蛋白相互作用在心脏和骨骼肌发育中的作用
- 批准号:
8457086 - 财政年份:2009
- 资助金额:
$ 12.47万 - 项目类别:
Role of LEK and Rb Protein Interactions in Heart and Skeletal Muscle Development
LEK 和 Rb 蛋白相互作用在心脏和骨骼肌发育中的作用
- 批准号:
7584583 - 财政年份:2009
- 资助金额:
$ 12.47万 - 项目类别:
Role of LEK and Rb Protein Interactions in Heart and Skeletal Muscle Development
LEK 和 Rb 蛋白相互作用在心脏和骨骼肌发育中的作用
- 批准号:
7842480 - 财政年份:2009
- 资助金额:
$ 12.47万 - 项目类别:
Function of CMF1 Protein During Avian Myogenesis
CMF1 蛋白在禽类肌肉生成过程中的功能
- 批准号:
6930437 - 财政年份:2002
- 资助金额:
$ 12.47万 - 项目类别:
Function of CMF1 Protein During Avian Myogenesis
CMF1 蛋白在禽类肌肉生成过程中的功能
- 批准号:
6439353 - 财政年份:2002
- 资助金额:
$ 12.47万 - 项目类别:
Function of CMF1 Protein During Avian Myogenesis
CMF1 蛋白在禽类肌肉生成过程中的功能
- 批准号:
6666923 - 财政年份:2002
- 资助金额:
$ 12.47万 - 项目类别:
Function of CMF1 Protein During Avian Myogenesis
CMF1 蛋白在禽类肌肉生成过程中的功能
- 批准号:
6788750 - 财政年份:2002
- 资助金额:
$ 12.47万 - 项目类别:
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