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Role of LEK and Rb Protein Interactions in Heart and Skeletal Muscle Development

LEK 和 Rb 蛋白相互作用在心脏和骨骼肌发育中的作用

基本信息

批准号：
8271375
负责人：
MARY Ellen DEES
金额：
$ 37.99万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-15 至 2014-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8271375
关键词：
Abnormal Cell Adult Affect Binding Binding Sites Birds CENPF gene Cardiac Cardiac Myoblasts Cardiac Myocytes Cardiomyopathies Cell Cycle Regulation Cell Differentiation process Cell Line Cell Proliferation Cell division Cells Complex Congenital Abnormality Critical Pathways Data Defect Development Differentiation and Growth Dilated Cardiomyopathy Embryo Embryonic Heart Ensure Equilibrium Exhibits Family member Future Goals Growth Healed Heart Heart Diseases Human In Vitro Injury Knock-out Leucine Life Light Mediating Modeling Mus Muscle Muscle Cells Muscle Development Muscle Fibers Mutation Myocardial Infarction Myocardium Neonatal Nuclear Organ Pathway interactions Phase Process Proliferating Property Protein Binding Protein Family Proteins Retinoblastoma Retinoblastoma Protein Role Set protein Site Skeletal Muscle Skeletal Myoblasts Skeletal system Testing Time Tissues Transgenic Animals Tumor Suppressor Proteins blastomere structure cell growth cell type congenital heart disorder design embryo tissue embryonic stem cell healing in vivo interest mouse model myogenesis null mutation prevent protein expression protein function stem cell therapy tool

项目摘要

We are interested in the processes which control cell division and differentiation in the developing embryo. These processes are dependent on the type of embryonic tissue, and must be tightly regulated to ensure the proper size, proportion, and function of each organ. In some cell types, such as skeletal muscle, cell division and differentiation are mutually exclusive: cells first proliferate (by dividing), then cease and initiate differentiation into mature muscle cells. In others, such as cardiac muscle, proliferation and differentiation occur together for a finite period of time. In the adult heart, however, once cells stop proliferating they have extremely limited capacity to resume. In the case of cardiac injury, for example in myocardial infarction or cardiomyopathy, this significantly limits the heart's ability to heal. In contrast, adult skeletal muscle heals readily in the case of injury. These differences in the adult tissue are set in embryonic life. We have identified a family of proteins, the LEK proteins, which appear to be important in the interplay between proliferation and differentiation in both embryonic heart and skeletal muscle. When we prevent mouse LEK protein from being made in a developing heart, the resulting heart is small and thin walled. This effect is intriguing in light of the functions we have demonstrated for LEK proteins in muscle cell proliferation and differentiation. Thus, our model of LEK disruption will be a valuable tool in the study of these processes in the heart. Further, we have identified a role of LEK proteins in a pathway critical for regulating growth and differentiation in all cells, the retinoblastoma protein (Rb) pathway. The Rb proteins are best known as tumor suppressors, that is for their role in controlling abnormal cell proliferation, but they also affect differentiation. We propose to further study the relationship between LEK and Rb proteins, and how they relate to another set of proteins well known to interact with Rb, the E2F proteins. The studies we have designed take advantage of mutations we have created in LEK proteins and expressed in heart and in skeletal muscle cells. With these tools, we can better understand fundamental defects of embryonic cardiac growth related to congenital heart disease. This can direct future therapy, such as stem cell therapy, to someday correct congenital defects at their origin and even stimulate damaged cardiac muscle to heal after injury such as myocardial infarction.

我们对控制细胞分裂和分化的过程感兴趣