Rapid Detection of Major Food-borne Pathogens

快速检测主要食源性病原体

• 批准号: 6644564
• 负责人: Peixuan Zhu
• 金额: $ 21.17万
• 依托单位: CREATV MICROTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6644564
• 关键词: Escherichia coli 0157:H7; Salmonella; Salmonella food poisoning; bacterial food poisoning; biohazard detection; biomedical equipment development; biosensor device; diagnosis quality /standard; food contamination; food poisoning; rapid diagnosis

DESCRIPTION (provided by applicant): Our food is a major source of illness. The Centers for Disease Control (CDC) estimates that food-borne diseases cause approximately 76 million incidents of illness, resulting in 325,000 hospitalizations and 5,000 deaths annually in the U.S. Known pathogens were implicated in 14 million of these incidents, 60,000 associated hospitalizations, and 1,800 deaths. Four pathogens alone (E. coli, Salmonella, Listeria, and Campylobacter) are believed to account for over two-thirds of deaths caused by known pathogens. The goal of our research is to achieve rapid, sensitive, and simple detection of pathogenic bacteria and toxins commonly found in foods by applying a new, very sensitive technology known as the "Integrating Waveguide Biosensor". This technology was recently developed by the Naval Research Laboratory (NRL) and is being licensed to Creatv MicroTech for application in the fields of water and food safety testing. NRL's initial experimental results for two molecules showed the Integrating Waveguide Biosensor to be 100 times more sensitive than the previous generation of biosensors based on optical fibers and planar arrays. We expect to achieve a similar improvement in sensitivity for detection of pathogens in food in a test that can be completed in less than 30 minutes. The resulting device will be ideally suited to the prevention of food-borne diseases. The initial scope in Phase I will focus on E. coli O157:H7 and Salmonella bacteria in ground beef and apple juice. A test instrument will be constructed incorporating the biosensor technology, assays will be developed and verified for the specified pathogens, and tests performed on food samples. In Phase II the scope will be expanded to include the pathogens Listeria monocytogenes and Campylobacter jejuni and the food groups poultry and fresh produce. The instrument will be redesigned to be more compact and portable, and assays developed for use outside a laboratory setting. Tests will be performed on location where these foods are produced, transported and/or prepared.

描述（由申请人提供）：我们的食物是主要的疾病来源。疾病控制中心（CDC）估计，食物传播的疾病造成约7600万例疾病，每年在美国已知的325,000例住院和5,000例死亡，其中1400万事件，60,000例相关住院和1,800人死亡。据信，单独使用四种病原体（大肠杆菌，沙门氏菌，李斯特菌和弯曲杆菌），占已知病原体引起的死亡的三分之二以上。 我们研究的目的是通过应用一种新的，非常敏感的技术（称为“整合波导生物传感器”）来实现食物中常见的致病细菌和毒素的快速，敏感和简单检测。这项技术最近是由海军研究实验室（NRL）开发的，并已获得许可以在水和食品安全测试领域中创建微技术。 NRL针对两个分子的初始实验结果表明，基于光纤和平面阵列的上一代生物传感器的整合波导生物传感器比上一代生物传感器高100倍。我们期望在不到30分钟内完成的测试中在食物中检测病原体检测的敏感性类似。最终的装置将非常适合预防食源性疾病。 第一阶段的初始范围将重点放在大肠杆菌O157：H7和碎牛肉和苹果汁中的沙门氏菌细菌上。将构建一种结合生物传感器技术的测试仪器，为指定病原体开发和验证测定，并对食物样品进行测试。在第二阶段中，范围将扩大，包括病原体单核细胞增生李斯特菌和弯曲杆菌的空肠，以及食物组家禽和新鲜农产品。该仪器将被重新设计为更紧凑和便携，并开发用于实验室环境外的测定法。测试将在生产，运输和/或准备的这些食物的位置进行。

项目成果

Detection of E. coli O157:H7 by immunomagnetic separation coupled with fluorescence immunoassay.

• DOI: 10.1016/j.bios.2011.09.029
• 发表时间: 2011-12
• 期刊: Biosensors & bioelectronics
• 影响因子: 12.6
• 作者: P. Zhu;D. Shelton;Shuhong Li;D. Adams;J. Karns;Platte T. Amstutz;Cha-Mei Tang

Detection of water-borne E. coli O157 using the integrating waveguide biosensor.

• DOI: 10.1016/j.bios.2005.01.005
• 发表时间: 2005-10
• 期刊: Biosensors & bioelectronics
• 影响因子: 12.6
• 作者: P. Zhu;D. Shelton;J. Karns;A. Sundaram;Shuhong Li;P. Amstutz;Cha-Mei Tang