Development of Vaccine for Neisseria gonorrhoeae

淋病奈瑟菌疫苗的研制

• 批准号: 6931513
• 负责人: Peixuan Zhu
• 金额: $ 35.55万
• 依托单位: CREATV MICROTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6931513
• 关键词: Escherichia coli; Neisseria gonorrhoeae; active immunization; adhesin; affinity chromatography; antibody; bacterial genetics; cell line; clinical research; functional /structural genomics; gene expression; gene mutation; genetic strain; gonorrhea vaccine; host organism interaction; humoral immunity; immune response; laboratory mouse; molecular cloning; mucosal immunity; nucleic acid sequence; polymerase chain reaction; recombinant proteins; vaccine development

DESCRIPTION (provided by applicant): Neisseria gonorrhoeae, a pathogenic agent of gonorrhoeae, is the major cause of sexually transmitted diseases (STD) in the United States, and there is currently no effective vaccine. Previous investigations using several cell surface components have failed in the developing of gonococcal vaccine due to the common drawback of antigenic variability and phase variation. Recently, an important virulence gene encoding the outer membrane adhesin/invasin protein has been discovered in the genome of pathogenic N. gonorrhoeae, which provide potential possibility to develop an effective vaccine against gonococcal diseases. This project is to study the vaccine potential of this selected protein from N. gonorrhoeae. This project will (1) evaluate the sequence variation of this gene of diverse N. gonorrhoeae strains from different sources, (2) investigate the immunogenicity of its recombinant protein and biological function of this gene, and (3) demonstrate if antibodies raised from this protein confer to protective immunity against gonococcal diseases. Several techniques will be used in this project including PCR, nucleotide sequencing, gene cloning and expression, gene knocking out, animal immunization, bactericidal and adhesion assays. The data from this project will provide fundamental information for understanding the role of this protein in N. gonorrhoeae and the possibility to develop a gonococcal vaccine.

描述（由申请人提供）：淋病的致病药物Neisseria Gonorrhoeae是美国性传播疾病（STD）的主要原因，目前没有有效的疫苗。由于抗原变异性和相变的共同缺点，使用几个细胞表面成分的先前研究在淋球菌疫苗的发展中失败了。最近，已经在致病性N. gonorrhoeae的基因组中发现了一个重要的毒力基因，该基因编码了外膜粘附素/侵入蛋白蛋白，该基因组提供了针对淋球菌疾病的有效疫苗的潜在可能性。 该项目是为了研究该选择蛋白质的疫苗潜力。该项目将（1）评估该基因从不同来源的多种淋病菌株的序列变化，（2）研究其重组蛋白和该基因的生物学功能的免疫原性，（3）证明是否从这种蛋白质中促进了这种蛋白质为抗蛋白质的抗体抗体抗体，以保护抗糖瘤抗体抗体。该项目将使用几种技术，包括PCR，核苷酸测序，基因克隆和表达，敲除基因，动物免疫，杀菌和粘附测定法。该项目的数据将提供基本信息，以了解该蛋白在淋病链球菌中的作用以及开发淋球菌疫苗的可能性。