CHEMICAL SENSORS FOR CELLULAR CONDITIONS AND COMPONENTS

用于细胞状况和成分的化学传感器

• 批准号: 6816146
• 负责人: Timothy Edward Glass
• 金额: $ 8.64万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6816146
• 关键词: chemical synthesis; excitatory aminoacid; intracellular; neurochemistry; oxidation reduction reaction; reagent /indicator

A general approach to modular, allosteric chemosensors is described. Chemosensors are small synthetic molecules which produces a visible signal upon interaction with a specific analyte. In the biochemical community, they have been used as sensitive, non-destructive methods for quantifying the amount of a particular analyte in cells. Chemosensors have played a pivotal role in unraveling the cellular function of a number of metal ions, most notably cell calcium. Similar sensors for organic molecules have not reached this level of success. The specific aims of this research are to develop a novel chemosensor framework and subsequently apply it to two specific problems. The first problem is the recognition of amino acids in aqueous solution. This is an important unsolved problem with broad ramifications. Specifically, excitatory amino acids will be targeted. This work will have direct impact on neurochemical research in that it is currently difficult to directly detect neurotransmitters such as glutamate in a non-destructive manner. Chemosensors for these molecules will enable a great deal of basic research into neurotransmission. The second specific target is a chemosensor for the redox potential present inside of cells. Since it has recently been suggested that a number of cell processes are controlled by the cellular redox potential, knowledge of this potential is of great importance. Sensors which can quickly and easily quantify the cellular reduction potential would be invaluable for work on the mechanisms of these processes.

描述了模块化变构化学传感器的一般方法。 化学传感器是小合成分子，可产生可见光 与特定分析物相互作用时发出信号。在生化方面 社区，它们已被用作敏感的、非破坏性的方法 量化细胞中特定分析物的量。化学传感器 在揭示细胞功能方面发挥了关键作用 金属离子的数量，最显着的是细胞钙。类似的传感器 有机分子尚未达到这种成功水平。具体的 这项研究的目的是开发一种新颖的化学传感器框架 随后将其应用于两个具体问题。第一个问题是 识别水溶液中的氨基酸。这是一个重要的 具有广泛影响的未解决问题。具体来说，兴奋 氨基酸将成为目标。这项工作将直接影响 神经化学研究目前很难直接 以非破坏性方式检测谷氨酸等神经递质。 这些分子的化学传感器将使大量的基础研究成为可能。 研究神经传递。第二个具体目标是 用于检测细胞内部氧化还原电位的化学传感器。自从它 最近有人提出，许多细胞过程 由细胞氧化还原电位控制，了解该电位 是非常重要的。可以快速、轻松地量化的传感器 细胞减少的潜力对于研究工作来说是无价的 这些过程的机制。