Chemical Sensors for Cellular Conditions and Components

用于细胞状况和成分的化学传感器

• 批准号: 6966683
• 负责人: Timothy Edward Glass
• 金额: $ 29.27万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6966683
• 关键词: aspartate; biosensor device; biotechnology; catecholamines; cell biology; cell components; chemical synthesis; excitatory aminoacid; fluorescent dye /probe; glutamates; ionophores; lysine; molecular probes; neurotransmitters; organic chemicals; oxidation reduction reaction; reagent /indicator

DESCRIPTION (provided by applicant): Fluorescent chemical sensors for small organic compounds will be developed. Chemical sensors are synthetic probes which produce a visible signal upon interaction with a specific analyte. In the biochemical community, these probes have been used as sensitive, nondestructive methods for quantifying the concentration of a particular analyte in cells. Chemical sensors have played a pivotal role in unraveling the cellular function of a number of metal ions, most notably cell calcium. Similar fluorescent sensors for organic molecules have not reached this level of success. The specific aims of this research are to develop novel chemical sensors for neurotransmitters and neuromodulators. This is an important problem with broad ramifications. This project will produce sensors for glutamate, aspartate, lysine, and the catecholamines which function under physiologically relevant conditions of pH and salinity. Furthermore, fluorophores which are pH dependant will be produced to expand the utility of this class of sensors. Biological studies will be performed with the aid of collaborators in which cells will be stained with the fluorescent sensors and the location and concentration of neurotransmitter will be measured.

描述（申请人提供）：将开发用于小有机化合物的荧光化学传感器。化学传感器是与特定分析物相互作用时产生可见信号的合成探针。在生物化学领域，这些探针已被用作敏感的、非破坏性的方法来定量细胞中特定分析物的浓度。化学传感器在揭示许多金属离子的细胞功能方面发挥了关键作用，其中最著名的是细胞钙。用于有机分子的类似荧光传感器还没有达到这种成功的程度。本研究的具体目的是为神经递质和神经调节剂开发新型化学传感器。这是一个影响广泛的重要问题。该项目将生产谷氨酸、天冬氨酸、赖氨酸和儿茶酚胺传感器，这些传感器在pH和盐度的生理相关条件下起作用。此外，将产生与pH值相关的荧光团，以扩大这类传感器的效用。生物学研究将在合作者的帮助下进行，细胞将被荧光传感器染色，神经递质的位置和浓度将被测量。