FLUORESCENT SENSORS FOR DETECTION OF BIOLOGICALLY IMPORTANT ORGANIC COMPOUNDS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本课题组一直致力于水介质中重要有机化合物荧光传感器的制备。荧光传感器是在目标分子（分析物）存在下产生可见荧光的化合物。这样的传感器已经被用于视觉追踪细胞中和周围的某些分析物的存在。这些研究已被证明是非常宝贵的细胞机制的阐明，通过提供实时信息的环境中的一个细胞在一个非破坏性的方式。然而，用于有机化合物的生物有用的传感器已经落后于用于金属离子和细胞条件（例如pH和pO 2）的传感器。制备有机化合物传感器的主要挑战是在细胞的复杂介质中获得感兴趣的分析物的特异性、高亲和力识别

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our group has been involved in the preparation of fluorescent sensors for detection of biologically important organic compounds in aqueous media. Fluorescent sensors are compounds which produce visible fluorescence in the presence of a target molecule (analyte). Such sensors have been used to visually trace the presence of certain analytes in and around cells. These studies have proven to be invaluable for the elucidation of cellular mechanisms by giving real-time information about the environment of a cell in a non-destructive manner. However, biologically useful sensors for organic compounds have lagged behind those for metal ions and cellular conditions such as pH and pO2. The main challenge in preparing sensors for organic compounds is obtaining specific, high affinity recognition of the analyte of interest in the complex media of the cell