New Model for Assessing Cartilage Repair and Protection

描述（由申请人提供）：从软骨移植到药物的各种方式已证明作为改变骨关节炎进展的方法具有相当大的前景。这导致了 对评估早期骨关节炎软骨的方法产生了浓厚的兴趣 人类和动物都会发生变化。该提案的目的是 改进当前的动物模型以评估旨在 治疗骨关节炎软骨。 具体来说，这项工作的假设是光学相干断层扫描 （OCT）与兔和大鼠骨关节炎模型结合时，将 在评估潜在疗法方面表现出独特的优势。 该计划是双方持续合作的延续 布莱根妇女医院和国王学院的研究人员。它是 假设所提出的模型除了增加关于 治疗的功效将减少需要治疗的动物数量 牺牲、需要处理的组织数量以及 需要治疗。此外，技术进步可以直接 转移到人体体内应用。本提案的假设将 通过技术开发和动物研究进行测试。具体细节 目标是： 目标 1. 构建一个等速偏振旋转器 华侨城系统。目标 2. 开发一种能够进入关节的 OCT 关节镜 通过 20 号针连接。目的。 3. 验证注册 基于关节镜的系统。目标 4. OCT 系统将在兔子身上进行测试 模型。目标 5. 将开发一种导管以在大鼠模型中成像。目标 6. OCT 成像导管将在大鼠模型中进行测试。

DESCRIPTION (Provided by Applicant): A variety of modalities, ranging from cartilage transplant to pharmaceuticals, have demonstrated considerable promise as methods for altering the progression of osteoarthritis. This has resulted in heightened interest in methods for assessing early osteoarthritic cartilage changes both in humans and animals. The objective of this proposal is to improve current animal models for the evaluation of therapeutics designed to treat osteoarthritic cartilage. Specifically, the hypothesis of this work is that Optical Coherence Tomography (OCT), when combine with rabbit and rat models of osteoarthritis, will demonstrate unique advantages for the evaluation of potential therapeutics. This program is a continuation of an ongoing collaboration between investigators at Brigham and Women's Hospital and King's College. It is postulated that the proposed model, in addition to increasing information on the efficacy of therapeutics, will reduce the number of animals that need to be sacrificed, the quantity of tissue requiring processing, and the amount of therapeutics required. Furthermore, technological advances can be directly transferred to in vivo human applications. The hypothesis of this proposal will be tested both through technology development and animal studies. The specifics aims are: Aim 1. To construct a constant velocity polarization rotator for the OCT system. Aim 2. To development an OCT arthroscope capable of accessing a joint through a 20 gauge needle. Aim. 3. To validate registration of the arthroscopic based system. Aim 4. The OCT system will be tested in a rabbit model. Aim 5. A catheter will be developed to image in a rat model. Aim 6. The OCT imaging catheter will be tested in a rat model.