TUMOR SUPPRESSOR GENE AT 13Q21 IN PROSTATE CANCER

2021 年第 13 季度前列腺癌中的肿瘤抑制基因

• 批准号: 6655056
• 负责人: JIN-TANG DONG
• 金额: $ 23.94万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-18 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6655056
• 关键词: apoptosis; artificial chromosomes; athymic mouse; carcinogenesis; cell line; cell proliferation; clinical research; complementary DNA; gene deletion mutation; genetic polymorphism; homozygote; human subject; loss of heterozygosity; male; neoplasm /cancer genetics; polymerase chain reaction; prostate neoplasms; sequence tagged sites; single strand conformation polymorphism; tumor suppressor genes

DESCRIPTION (Adapted from investigator's abstract): Prostate cancer has a complex pathogenesis in which many men develop early stage 'benign' cancers, and a much smaller but substantial number develop aggressive cancers that metastasize and cause premature death. The PI has identified a region on chromosome 13q21 which appears to contain a gene whose alteration contributes to the aggressive behavior of prostate cancer. This region is subject to LOH and homozygous deletion in aggressive prostate cancers and cell lines. The current minimal region contains at least three genes which are expressed in normal prostate and low-grade tumors, but not aggressive cancers. The PI now proposes to define the smallest region of deletions that contains the 13q21 gene; to identify the 13q21 tumor suppressor gene (TSG) through expression and mutation analyses; to express the gene in cancer cell lines to examine effects on growth and metastasis; and examine in greater detail how often LOH and homozygous deletion affect the gene, the expression of the gene in prostate cancer, and clinical and pathologic correlations including prognostic significance.

描述(改编自研究人员摘要)：前列腺癌 许多男性发展为早期良性癌症的复杂发病机制， 数量少得多但相当多的人会患上侵袭性癌症 转移并导致过早死亡。公安局已经确定了一个区域 染色体13q21似乎包含一个基因，该基因的改变有助于 前列腺癌的侵袭性行为。这一地区受到陆上交通事故的影响 以及侵袭性前列腺癌和细胞系中的纯合子缺失。这个 当前最小区域包含至少三个基因，这些基因在 正常的前列腺癌和低级别肿瘤，但不是侵袭性癌症。私家侦探现在 建议定义包含13q21的最小缺失区域 通过表达和鉴定13q21抑癌基因(TSG) 突变分析；在癌细胞系中表达该基因以检验效果 对生长和转移的影响；并更详细地检查LOH和 纯合子缺失影响该基因，该基因在前列腺中的表达 癌症与临床和病理的相关性，包括预后 意义。