Bone Lead Standardization Program

描述(由申请人提供)：本提案的目的是创建通过参考方法和活体X射线荧光(XRF)获得的骨铅测量(BLSP)的标准化计划。BLSP将满足骨铅方法学目前的需求：缺乏适当的认证标准物质(RMS)和缺乏可追溯性。BLSP还将为电热原子吸收光谱分析(AAS)和电感耦合等离子体质谱(ICPMS)提供RMS，并将使用这些方法在实验室之间进行实验室间验证研究。该计划还将为XRF提供RMS，从而提供SI单位的可追溯性，并将在使用XRF进行活体骨铅测量的实验室之间进行实验室间验证研究。该中心有六个目标：第一，在纽约州卫生局沃兹沃斯微量元素实验室(NYS DOH)交叉验证原子吸收光谱和电感耦合等离子体质谱；第二，建立可追溯到NIST SRM 1486骨粉和1400骨灰的国家级RMS骨铅测量资料库；第三，对AAS和/或电感耦合等离子体质谱进行骨铅测量的实验室间验证。第四个目标是通过制备两套特征良好的羊骨样本来组装骨铅XRF的国家级RMS储存库；第五个目标是使用这两组羊骨样本进行体外XRF实验室间验证和比较研究。第一套XRF RMS将被销毁，作为通过原子吸收光谱和电感耦合等离子体质谱获得可追溯到SI单位的表面和核心骨的参考浓度过程的一部分。由此获得的参考浓度将构成XRF准确度评估的基础。第二套XRF RMS将被创建并用于第二次实验室间比对，并将被保存以供长期使用。第六个也是最后一个目标是使用将提供RMS的NYS DOH山羊群体的安乐死成员进行体内XRF验证研究。体内测量的XRF浓度将与通过原子吸收光谱分析和电感耦合等离子体质谱获得的参考浓度进行比较。

DESCRIPTION (provided by applicant): The aim of this proposal is to create a Standardization Program for Bone Lead measurements (BLSP) obtained via reference methods and via in vivo X-Ray Fluorescence (XRF). The BLSP will fulfill current needs of the bone lead methodologies: lack of appropriate certified reference materials (RMs) and lack of traceability. The BLSP will also provide RMs for electrothermal atomic absorption spectrometry (AAS) and inductively coupled plasma-mass spectrometry (ICP-MS) and will conduct an interlaboratory validation study among laboratories using those methods. The program will also provide RMs for XRF, thereby providing traceability to SI units, and will conduct an interlaboratory validation study among laboratories using XRF for in vivo bone lead measurements. There are six goals: first to cross-validate AAS and ICP-MS in the Wadsworth Trace Elements Laboratory of the New York State Department of Health (NYS DOH); second to establish a national repository of RMs for bone lead measurement via AAS and ICP-MS traceable to NIST SRM 1486 Bone Meal and 1400 Bone Ash; third, to conduct an interlaboratory validation of AAS and/or ICP-MS for bone lead measurement. The fourth goal is to assemble a national repository of RMs for bone lead XRF by preparing two sets of well-characterized goat bone samples containing endogenous lead concentrations that encompass those found in human populations; the fifth goal is to conduct an in vitro XRF interlaboratory validation and comparison study using the two sets of goat bone samples. The first set of XRF RMs will be destroyed as part of the process of obtaining reference concentrations via AAS and ICP-MS for both surface and core bone that are traceable to SI units. The reference concentrations thus obtained will form the basis of the assessment of XRF accuracy. The second set of XRF RMs will be created and used for a second interlaboratory comparison and will be preserved for long-term use. The sixth and last goal is to conduct an in vivo XRF validation study using the euthanized members of the NYS DOH goat colony that will provide the RMs. XRF-measured in vivo concentrations will be compared to the reference concentrations obtained from AAS and ICP-MS.