BORNA VIRUS ALTERS PERINATAL BEHAVIOR DEVELOPMENT

病毒感染发育中的大脑是一种严重而常见的原因 儿科神经系统疾病。因为大脑在继续发育 出生后，中枢神经系统(CNS)可能会受到损伤 在出生前、围产期和产后感染病毒之后。艾滋病毒引发一起 最常见的由病毒引起的儿童神经系统疾病 美国。值得注意的是，尽管发育迟缓的临床证据 神经系统疾病是感染艾滋病毒的儿童中的一个突出发现， 病毒相关性神经发育损伤机制的研究进展 人们对此了解甚少。博尔纳病病毒感染的研究 新生的Lewis大鼠揭示了关于 神经解剖学和神经行为学对脑发育的影响 脑部持续的病毒感染。这个动物模型系统是 有助于发育性神经病学发病机制的研究 围产期感染艾滋病毒后的疾病。 在特定目标1中，BDV对大鼠造成发育损伤 小脑将通过小脑的定量测量来测量 大小和皮质发育与小脑和海马齿状回 随着时间的推移，脑回神经元存活。在机械分析中，我们将 确定重要的发育基因mRNA表达的变化 围产期病毒感染后的小脑。《特定目标2》探索 感觉运动、情绪发育的相关异常 神经递质的认知、社会行为和异常 病毒后感染脑组织中的浓度与时间的关系 感染。这些多维神经解剖学的结果/ 神经行为发病机制的研究将促进我们对 病毒对婴儿大脑发育的影响，以及解剖学上的 围产期感染后的行为病理学联系 持久性RNA病毒。

Virus infections of the developing brain are a serious and common cause of pediatric neurological disease. Since the brain continues to develop after birth, central nervous system (CNS) injuries can be sustained following pre-, peri- and post-natal virus infections. HIV causes one of the most common virus-induced neurological diseases of children in the US. Notably, although clinical evidence of developmental neurological disease is a prominent finding in HIV infected children, the mechanisms of the virus-related neurological developmental injury are poorly understood. Studies of Borna disease virus (BDV)-infected neonatal Lewis rats have revealed important principles about the neuroanatomical and neurobehavioral effects on brain development of persistent virus infections of the brain. This animal model system is useful for the study the pathogenesis of developmental neurological diseases following perinatal HIV infection. In Specific Aim 1, BDV-induced developmental damage to the rat cerebellum will be measured via quantitative measurements of cerebellar size and cortical development and cerebellar and hippocampal dentate gyrus neuron survival over time. In a mechanistic analysis, we will identify changes in important developmental gene mRNA expression in the cerebellum following perinatal virus infection. Specific Aim 2 explores associated abnormalities in the development of sensorimotor, emotion, cognitive, and social behaviors and abnormalities in neurotransmitter concentrations in the infected brain as a function of time post virus infection. The results of these multidimensional neuroanatomical/ neurobehavioral pathogenesis studies will advance our knowledge of the effects of viruses on infant brain development, and of anatomical- behavioral pathology linkages following perinatal infection with a persistent RNA virus.