PROLACTIN EFFECTS ON BEHAVIOR

催乳素对行为的影响

• 批准号: 6638976
• 负责人: JOHN D BUNTIN
• 金额: $ 17.99万
• 依托单位: UNIVERSITY OF WISCONSIN MILWAUKEE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6638976
• 关键词: Aves; behavioral /social science research tag; dopamine; eating; ethology; gene expression; glucocorticoids; gonadotropin releasing factor; hormone regulation /control mechanism; injection /infusion; maternal behavior; neuroanatomy; neurochemistry; neuroendocrine system; neuropeptide Y; nutrient intake activity; paternal behavior; prolactin; regulatory gene

DESCRIPTION (adapted from applicant's abstract): Prolactin (PRL) promotes and coordinates the various physiological and behavioral changes that are necessary for successful parental care in vertebrates. Although the effects of PRL on peripheral target organs that serve parental functions have been studied in some depth, much less is known about how PRL acts directly or indirectly on the brain to induce parental behavior and associated neuroendocrine changes, such as reduced gonadal activity. This proposal employs a powerful avian model, the ring dove (Streptopelia risoria), to explore these effects in detail. The proposed studies build on our previous work to 1) characterize the neurochemical events that mediate these changes and 2) identify key components of the neural circuitry involved. Neurochemical investigations will evaluate neuropeptide Y (NPY) and adrenal glucocorticoids as potential mediators of PRL-induced parental hyperphagia (feeding) and gonadal suppression. These studies will examine the effects of intracerebroventricular (ICV) injections of these compounds and measure changes in NPY gene expression and protein in response to PRL or glucocorticoid injection. They will also determine if these PRL-induced changes are attenuated by blocking NPY or glucocorticoid action. Studies of possible GnRH mediation of PRL-induced gonadal suppression and dopamine involvement in PRL-induced parental hyperphagia are also proposed. Neuroanatomical investigations will use immunocytochemical detection of immediate-early gene products to identify neurons that are activated in response to ICV injection of PRL, NPY, and glucocorticoids, to visualize the pattern of neuronal activation associated with parental interactions with young, and to determine how the pattern changes in response to PRL and previous parental experience. These studies contribute to the long term objective of obtaining an integrated picture of how PRL influences brain function and behavior in a parental context. They will also increase our limited understanding of how adenohypophyseal hormones influence brain activity and provide new insights into the causes of infertility, energy balance, and behavioral alterations that are associated with hyperprolactinemia in the clinical setting.

描述（改编自申请人的摘要）：催乳素（PRL）促进和 协调各种必要的生理和行为变化 成功地对脊椎动物进行亲代照顾。虽然 PRL 对 服务亲代功能的外周靶器官已被研究 对于 PRL 如何直接或间接作用于 大脑诱导父母行为和相关的神经内分泌变化，例如 由于性腺活动减少。该提案采用了强大的鸟类模型， 环鸠（Streptopelia risoria），详细探讨这些影响。这 拟议的研究建立在我们之前的工作的基础上：1）描述 介导这些变化的神经化学事件以及 2) 确定关键成分 所涉及的神经回路。神经化学研究将评估 神经肽 Y (NPY) 和肾上腺糖皮质激素作为潜在介质 PRL 诱导的父母食欲亢进（喂养）和性腺抑制。这些 研究将检查脑室内（ICV）注射的效果 这些化合物并测量 NPY 基因表达和蛋白质的变化 对 PRL 或糖皮质激素注射的反应。他们还将确定这些是否 PRL 引起的变化可通过阻断 NPY 或糖皮质激素的作用而减弱。 GnRH 可能介导 PRL 诱导的性腺抑制的研究 还提出多巴胺参与 PRL 诱导的亲本食欲亢进。 神经解剖学研究将使用免疫细胞化学检测 立即早期基因产物来识别被激活的神经元 对 ICV 注射 PRL、NPY 和糖皮质激素的反应，以可视化 与父母互动相关的神经元激活模式 年轻，并确定模式如何响应 PRL 和以前的变化 父母的经历。这些研究有助于实现长期目标 全面了解 PRL 如何影响大脑功能并 父母背景下的行为。他们还将增加我们有限的 了解腺垂体激素如何影响大脑活动和 为不孕不育的原因、能量平衡和 与高催乳素血症相关的行为改变 临床环境。