PROLACTIN EFFECTS ON BEHAVIOR; SITES & MODES OF ACTION

催乳素(PRL)是一种脑下垂体激素，是一种可能的神经肽。 异常多样但鲜为人知的一系列行为行为。 拟议的研究将使用环鸽(Streptopelia Risoria)作为 探讨催乳素作用生理机制的模型系统 中枢神经系统(CNS)和n个外周靶组织 影响行为表达并确定他的激素在哪里起作用 大脑来促进这些变化，这些变化是中央调节的。戒指 DOVE非常适合于这些研究，因为1)各种PRL诱导的 在这个物种中已经记录了行为变化，2)PRL行为 在中心和外围位置，以唤起这些不同的行为 变化，以及3)催乳素敏感靶细胞的分布 精确地绘制在环鸽的大脑中。拟议的研究将 跟进之前由NIMH赞助的表征研究 实现五个具体目标：1)确定四个对催乳素敏感的角色 鸽子脑内促进催乳素诱导的摄食行为的区域 和抑制促性腺激素的分泌(这反过来可能会 行为后果)；2)更准确地定义 鸽子中枢神经系统内调节催乳素和生长的受体种群(S) 激素引起的摄食和促性腺激素分泌的变化 这些荷尔蒙活动可以被模仿或阻止的程度 针对纯化的PRL受体的抗体；3)确定 催乳素引起的促性腺激素分泌的改变是由于垂体的改变 促性腺激素释放激素或促性腺激素释放激素改变的敏感性 分泌；4)确定催乳素作用于亲本的中枢神经系统部位 对年轻人的反应性，这一中央调节的特异性 在非神经靶组织中的作用以及催乳素诱导的改变的作用 在促进父母活动方面；5)确定脑室内 注射催乳素维持分离配偶的持续孵化行为 鸽子。这些研究是为将来的考试做准备 催乳素行为的神经化学基础及本地鸽子的作用 PRL在促进这一功能上的重大行为变化方面 物种。总的来说，这些实验应该有助于阐明 行为和神经内分泌的生理机制 与临床高催乳素血症相关的病理改变 蛋白质荷尔蒙行为基本原理的表述 互动。

Prolactin (PRL) is a pituitary hormone and putative neuropeptide with an exceptionally diverse but poorly understood array of behavioral actions. The proposed research will employ the ring dove (Streptopelia risoria) as a model system to explore he physiological mechanisms by which PRL acts in the central nervous system (CNS) and n peripheral target tissues to influence behavioral expression and to determine where he hormone acts in the brain to promote those changes that are centrally mediated. The ring dove is well suited for these studies because 1) a variety of PRL-induced behavioral alterations have been documented in this species, 2) PRL acts at both central, and peripheral sites to evoke these various behavioral changes, and 3) the distribution of PRL-sensitive target cells has been precisely mapped in the ring dove brain. The proposed research would follow up on previous NIMH-sponsored characterization studies to accomplish five specific aims: 1) to define the role of four PRL-sensitive regions within the dove brain in promoting PRL-induced feeding behavior and suppression of gonadotropin secretion (which in turn could have behavioral consequences); 2) to more precisely define the nature of the receptor population(s) within the dove CNS which mediate PRL and growth hormone-induced changes in feeding and gonadotropin secretion by testing the degree to which these hormone actions can be mimicked or blocked by antibodies against purified PRL receptors; 3) To determine whether PRL-induced changes n gonadotropin secretion are due to altered pituitary sensitivity to gonadotropin releasing hormone (GNRH) or altered GNRH secretion; 4) to define the CNS sites of PRL action in promoting parental responsiveness towards young, the specificity of this centrally mediated action, and the role of PRL-induced changes in non-neural target tissues in promoting parental activity; 5) to determine if intracerebroventricular injections of PRL maintain ongoing incubation behavior in mate-separated doves. These studies are preparatory to a future examination of the neurochemical basis of PRL's behavioral action and the role of native dove PRL in promoting functionally significant behavioral changes in this species. Collectively, these experiments should help elucidate the physiological mechanisms underlying the behavioral and neuroendocrine pathologies associated with clinical hyperprolactinemia and will aid in the formulation of general principles underlying protein hormone-behavior interactions.