PROLACTIN EFFECTS ON BEHAVIOR; SITES & MODES OF ACTION

催乳素对行为的影响；

• 批准号: 3486784
• 负责人: JOHN D BUNTIN
• 金额: $ 10.4万
• 依托单位: UNIVERSITY OF WISCONSIN MILWAUKEE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3486784
• 关键词: Aves; autoradiography; central nervous system; dosage; eating; ethology; gonadotropin releasing factor; growth factor; hormone receptor; hormone regulation /control mechanism; longitudinal animal study; luteinizing hormone; maternal behavior; neuroendocrine system; paternal behavior; preoptic areas; prolactin; radioimmunoassay; videotape /videodisc

Prolactin (PRL) is a pituitary hormone and putative neuropeptide with an exceptionally diverse but poorly understood array of behavioral actions. The proposed research will employ the ring dove (Streptopelia risoria) as a model system to explore he physiological mechanisms by which PRL acts in the central nervous system (CNS) and n peripheral target tissues to influence behavioral expression and to determine where he hormone acts in the brain to promote those changes that are centrally mediated. The ring dove is well suited for these studies because 1) a variety of PRL-induced behavioral alterations have been documented in this species, 2) PRL acts at both central, and peripheral sites to evoke these various behavioral changes, and 3) the distribution of PRL-sensitive target cells has been precisely mapped in the ring dove brain. The proposed research would follow up on previous NIMH-sponsored characterization studies to accomplish five specific aims: 1) to define the role of four PRL-sensitive regions within the dove brain in promoting PRL-induced feeding behavior and suppression of gonadotropin secretion (which in turn could have behavioral consequences); 2) to more precisely define the nature of the receptor population(s) within the dove CNS which mediate PRL and growth hormone-induced changes in feeding and gonadotropin secretion by testing the degree to which these hormone actions can be mimicked or blocked by antibodies against purified PRL receptors; 3) To determine whether PRL-induced changes n gonadotropin secretion are due to altered pituitary sensitivity to gonadotropin releasing hormone (GNRH) or altered GNRH secretion; 4) to define the CNS sites of PRL action in promoting parental responsiveness towards young, the specificity of this centrally mediated action, and the role of PRL-induced changes in non-neural target tissues in promoting parental activity; 5) to determine if intracerebroventricular injections of PRL maintain ongoing incubation behavior in mate-separated doves. These studies are preparatory to a future examination of the neurochemical basis of PRL's behavioral action and the role of native dove PRL in promoting functionally significant behavioral changes in this species. Collectively, these experiments should help elucidate the physiological mechanisms underlying the behavioral and neuroendocrine pathologies associated with clinical hyperprolactinemia and will aid in the formulation of general principles underlying protein hormone-behavior interactions.

催乳素 (PRL) 是一种垂体激素和推定的神经肽，具有 异常多样化但人们知之甚少的一系列行为行为。 拟议的研究将采用环鸠（Streptopelia risoria）作为 探索 PRL 作用生理机制的模型系统 中枢神经系统 (CNS) 和周围靶组织 影响行为表达并确定激素作用的位置 大脑促进那些由中枢介导的变化。戒指 dove 非常适合这些研究，因为 1) 多种 PRL 诱导的 该物种的行为改变已被记录，2) PRL 行为 在中枢和外围部位唤起这些不同的行为 变化，以及3）PRL敏感靶细胞的分布已发生变化 精确地映射在环斑鸠的大脑中。拟议的研究将 跟进之前 NIMH 赞助的特征研究 实现五个具体目标：1）定义四种PRL敏感的作用 鸽子脑内区域促进 PRL 诱导的摄食行为 和抑制促性腺激素分泌（这反过来又可能 行为后果）； 2）更准确地定义事物的性质 鸽子 CNS 内介导 PRL 和生长的受体群 通过测试激素引起的进食和促性腺激素分泌变化 这些激素作用可以被模仿或阻断的程度 针对纯化的 PRL 受体的抗体； 3）判断是否 PRL 引起的促性腺激素分泌变化是由于垂体改变所致 对促性腺激素释放激素 (GNRH) 或 GNRH 改变的敏感性 分泌; 4) 确定 PRL 促进亲代作用的中枢神经系统位点 对年轻人的反应，这种中枢介导的特异性 PRL 诱导的非神经靶组织变化的作用和作用 促进家长活动； 5) 判断是否脑室内 注射 PRL 可维持配偶分离的持续孵化行为 鸽子。这些研究为未来的检查做准备 PRL 行为作用的神经化学基础和本地鸽子的作用 PRL 在促进功能上显着的行为改变方面 物种。总的来说，这些实验应该有助于阐明 行为和神经内分泌的生理机制 与临床高催乳素血症相关的病理学，将有助于 蛋白质激素行为的一般原则的制定 互动。