Acquisition of a Circular Dichroism Spectrometer

购买圆二色性光谱仪

• 批准号: 6439978
• 负责人: BARBARA M BRODSKY
• 金额: $ 16.17万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439978
• 关键词: biomedical equipment purchase; circular dichroism

This application is for a new circular dichroism (CD) spectrometer system, to replace the instrument obtained in 1991 through an NIH Shared Instrumentation Grant. A highly successful shared CD spectroscopy facility has been operating in Robert Wood Johnson Medial School for the past 10 years, under the administration of Dr. Norma Greenfield, an acknowledged expert in this field. The present CD instrument (an Aviv 62 DS provided a cutting edge research tool for studying protein and nucleic acid conformation, stability, and ligand binding to the laboratories of 5 NIH-funded major users, and more than 20 funded minor uses. CD studies from the facility have been included in more than 90 publications. In addition to its research productivity, the CD facility has served as a research-intensive learning environment. Dr. Greenfield has trained more than 100 graduate students, undergraduates, and post-doctoral researchers in the collection, analysis and interpretation of CD spectra of proteins and nucleic acids. For many of these trainees, this was their first exposure to structural biology methodology and analysis. This facility has been funded by user fees, which include 25% of Dr. Greenfield's salary, a service contract, and the nitrogen supply. The continued success of this multi-user facility is endangered. The DOS based computer operating system of the Aviv 62DS CD spectrometer is outdated and its interface boards are incompatible with Pentium computers. The optical performance of this instrument can no longer match that f the new generation of instruments. An Aviv Model 2156 CD instrument is requested in this application, together with a stopped low accessory and several recently developed attachments. This modern instrument and accessories will ensure the continued operation of a successful facility, which supports the research efforts of many funded investigators and complements other available biophysical techniques.

本申请是一种新的圆二色性（CD）光谱仪系统，以取代1991年通过NIH共享仪器赠款获得的仪器。一个非常成功的共享CD光谱设施一直在罗伯特伍德约翰逊医学院在过去的10年中，在诺玛格林菲尔德博士，在这一领域的公认专家的管理。目前的CD仪器（Aviv 62 DS）为5个NIH资助的主要用户的实验室和20多个资助的次要用途提供了研究蛋白质和核酸构象、稳定性和配体结合的尖端研究工具。该设施的CD研究已被收录在90多份出版物中。除了其研究生产力，光盘设施已作为一个研究密集型的学习环境。格林菲尔德博士在蛋白质和核酸的CD光谱的收集、分析和解释方面培训了100多名研究生、本科生和博士后研究人员。对于这些学员中的许多人来说，这是他们第一次接触结构生物学方法和分析。该设施的资金来自用户费，其中包括格林菲尔德博士工资的25%、服务合同和氮气供应。这一多用户设施的持续成功受到威胁。Aviv 62DS CD光谱仪基于DOS的计算机操作系统已经过时，其接口板与Pentium计算机不兼容。这台仪器的光学性能已不能与新一代仪器相比。本申请要求使用Aviv 2156型CD仪器，以及一个停止低附件和几个最近开发的附件。这一现代仪器和附件将确保一个成功的设施的持续运作，支持许多受资助的研究人员的研究工作，并补充其他现有的生物物理技术。