Small molecule ligand interactions

小分子配体相互作用

• 批准号: 6662100
• 负责人: DUANE D MILLER
• 金额: $ 18.34万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6662100
• 关键词: CD antigens; antiviral agents; astrocytes; cooperative study; drug discovery /isolation; hepatitis C; hepatitis C virus; intermolecular interaction; ligands; tissue /cell culture; virus infection mechanism

Hepatitis C virus (HCV) represents a major health problem for the world. It is a chronic disease that effects mainly the liver and currently there is no cure. Although interferon-alpha and ribavirin are the currently used therapy this approach has many shortcomings. The recent report that the HCV envelope E2 binds to the human CD81 tetraspanin protein has provided valuable new targets for the search for new agents that could block this initial binding of the HCV to human cells. CD81 (TAPA, Target of the Anti-Proliferative Antibody) is a 236-residue protein and a member of the tetraspanin family. The knowledge about CD81 and E2 has stimulated new approach of interfering with the HCV binding to human cells. Our objectives are to: 1- Identify small molecules that will bind to astrocytes containing CD81. Our hypothesis states we will identify new synthetic and natural substances that bind to CD81 and prevent the fusion of HCV with the human cells. Our approach will be to examine libraries of synthetic and natural products obtained in the United States and other countries for their ability to bind to CD81 and a 96 well high throughput assay system. 2- Identify compounds that will bind to the envelop proteins (E1 and E2) and inhibit or interfere with the binding and fusion of the HCV virus to human cells. We will identify compounds that inhibit the spread of a HCV surrogate virus (rVSV, contain envelope proteins, E1 and E2) in cell culture. 3- Model the interaction of CD81 protein and the envelope proteins (E1 and E2) and to show how molecules can interfere with this interaction. Insights gained from this work will aid in the design of new molecules that will be very specific in binding to CD81 or the envelope proteins. The viral attachment and entry in the viral life-cycle has proven to be an effective point to attack with other viruses such as influenza and HIV. Insights gained from modeling and lead compounds should lead to a highly effective treatment and or new methods for the prevention of the HCV infection.

丙型肝炎病毒（HCV）是世界上的一个主要卫生问题。这是一种慢性疾病，主要影响肝脏，目前还没有治愈方法。虽然干扰素- α和利巴韦林是目前使用的治疗方法，但这种方法有许多缺点。最近有报道称HCV包膜E2与人CD81四白蛋白结合，这为寻找能够阻断HCV与人细胞初始结合的新药物提供了有价值的新靶点。CD81 （TAPA, Target of the anti - prolifative Antibody）是一种236残基蛋白，是四蛋白家族的一员。对CD81和E2的了解激发了干扰HCV与人类细胞结合的新方法。我们的目标是：1-确定将与含有CD81的星形胶质细胞结合的小分子。我们的假设是，我们将发现新的合成物质和天然物质结合CD81，并阻止HCV与人类细胞的融合。我们的方法将是检查在美国和其他国家获得的合成和天然产物库，以确定它们与CD81结合的能力，并使用96孔高通量分析系统。鉴定能与包膜蛋白（E1和E2）结合并抑制或干扰HCV病毒与人类细胞结合和融合的化合物。我们将在细胞培养中确定抑制HCV替代病毒（rVSV，含有包膜蛋白E1和E2）传播的化合物。建立CD81蛋白与包膜蛋白（E1和E2）相互作用的模型，并展示分子如何干扰这种相互作用。从这项工作中获得的见解将有助于设计与CD81或包膜蛋白结合的新分子。病毒在病毒生命周期中的附着和进入已被证明是与其他病毒（如流感和艾滋病毒）攻击的有效点。从建模和先导化合物中获得的见解应该导致预防HCV感染的高效治疗和/或新方法。