Regulation of light sensitivity by phosducin & 14-3-3

光敏性的调节

• 批准号: 6623421
• 负责人: MARK W BITENSKY
• 金额: $ 36.25万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-10 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6623421
• 关键词: calcium channel; dark adaptation; electrophysiology; electroretinography; glutamates; laboratory rat; lens proteins; light adaptations; mass spectrometry; molecular cloning; neurotransmitter transport; phosphoproteins; phosphorylation; protein isoforms; protein protein interaction; retina; rod cell; second messengers; synaptosomes; tau proteins; voltage gated channel; western blottings

DESCRIPTION (provided by applicant): This application's long term research objective is to contribute new information that will strengthen understanding of' the critical process of light and dark adaptation in the visual system especially in the context of specific new findings on the light regulation of rod synapse proteins. A principle aim of this research will be to identify gene products with specific relevance for light adaptation and potential significance for understanding the genetics and molecular biology of human retinal diseases that endanger vision. The experiments focus on extending and explaining the observation of unforeseen light regulated interactions between Phosducin and 14-3-3 in the rod synapse. The power of the experiments is amplified by the combined use of ERG recordings and protein chemical analyses on the same experimental subject. The experiments are further enhanced by the confinement powers of the rod ribbon synaptosome which can safely shelter and deliver for analysis an undiluted, unmixed population of rod synapse gene products through the maelstrom of retinal homogenization. The program tests a new hypothesis with definitive experiments. It is proposed that the light regulated interactions between Phosducin and 14-3-3, both having the capability to regulate glutamate release in the rod synapse. contribute an important term in the equation of rod sensitivity control and in the flow of information between the rod photoreceptor cell, the bipolar cells and higher order retinal neurons. The proposed experiments are the fruits of a multidisciplinary collaboration involving Departments of Biomedical Engineering (BU), Neurophysiology (Northeastern), and Computational Biology (Weizmann Institute).

描述（申请人提供）：本申请的长期研究 目的是提供新的信息， 视觉系统中光暗适应的关键过程 特别是在特定的新发现的背景下， 杆突触蛋白。这项研究的主要目的是确定基因 与光适应和潜力特别相关的产品 对理解人类遗传学和分子生物学的意义 危及视力的视网膜疾病。实验的重点是扩展和 解释了观察到的不可预见的光调节相互作用， 视杆突触中的磷酸化蛋白和14-3-3。实验的力量在于 通过ERG记录和蛋白质化学分析的结合使用来放大 同一个实验对象。这些实验进一步得到加强， 限制权力的杆带突触体，可以安全地庇护， 将未稀释的、未混合的视杆细胞突触基因群体 通过视网膜同质化的漩涡。该程序测试了一个 新的假设和确定的实验。有人建议， 调节Phosducin和14-3-3之间的相互作用，两者都具有 来调节杆突触中谷氨酸的释放贡献一个重要的术语 在控制棒灵敏度方程和信息流中 视杆细胞、双极细胞和高级视网膜细胞之间的相互作用 神经元拟议的实验是一个多学科的成果， 涉及生物医学工程部门（BU）的合作， 神经生理学（东北）和计算生物学（魏茨曼研究所）。