Automated and Advanced Characterisation of Single Nanoparticles

单纳米颗粒的自动化和高级表征

• 批准号: 2270505
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2270505
• 关键词: Automated; Advanced; Characterisation; Single; Nanoparticles

Nanoparticles have revolutionised areas of medicine ranging from drug delivery to medical imaging to ultra-sensitive biosensors. However, many nanoparticles are still prepared with bulk synthesis methods, which leads to poor control over particle properties such as size and loading efficiency. This heterogeneity has significant biological consequences, which can severely impede the translation of nanomedicines to the clinic. Microfluidics offers a promising solution. The small volumes used in microfluidics offer improved control over mass transport compared to bulk approaches, allowing for more homogeneous nanoparticle physiochemical properties and higher batch-to-batch reproducibility.To assess inter-particle heterogeneity requires single particle characterisation techniques. One effective method is SPARTA, a recently developed, high-throughput and automated technique that uses Raman spectroscopy to non-destructively analyse single nanoparticles. This project will use SPARTA to assess the quality of various nanomedicines prepared with bulk and microfluidic methods. To provide complementary imaging information, samples will be characterised with liquid-phase electron microscopy, a technique capable of imaging samples in water with sub-nanometre resolution.This project will focus on techniques which are scalable to industry and materials which are previously approved for used in humans to improve the translational quality of the proposed technology.

纳米粒子已经彻底改变了医学领域，从药物输送到医学成像到超灵敏的生物传感器。然而，许多纳米颗粒仍然是通过本体合成方法制备的，这导致对颗粒性质（例如尺寸和负载效率）的控制较差。这种异质性具有显著的生物学后果，这可能严重阻碍纳米药物向临床的转化。微流体技术提供了一个很有前途的解决方案。微流体中使用的小体积提供了改进的控制质量传输相比，散装的方法，允许更均匀的纳米粒子的理化性质和更高的批次间reproducibility.To评估颗粒间的异质性需要单颗粒表征技术。一种有效的方法是斯巴达，这是一种最近开发的高通量自动化技术，使用拉曼光谱法对单个纳米颗粒进行非破坏性分析。该项目将使用斯巴达来评估用批量和微流体方法制备的各种纳米药物的质量。为了提供互补的成像信息，将使用液相电子显微镜对样品进行表征，这是一种能够以亚纳米分辨率对水中样品进行成像的技术。该项目将专注于可扩展到工业和材料的技术，这些技术先前已被批准用于人类，以提高拟议技术的转化质量。